{"product_id":"laboratory-methods-in-microbiology-and-molecular-biology-methods-in-molecular-microbiology-paperback-9780323950787","title":"Laboratory Methods in Microbiology and Molecular Biology; Methods in Molecular Microbiology (Paperback) 9780323950787","description":"\u003cfont face=\"Georgia\"\u003e\r\n\u003cp\u003e\u003cfont size=\"6\"\u003eLaboratory Methods in Microbiology and Molecular Biology\u003c\/font\u003e\u003cbr\u003e\r\n\u003cfont size=\"5\"\u003eMethods in Molecular Microbiology\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cem\u003eA refreshing update of current practice and future prospects for several important areas in the field of microbial biotechnology\u003c\/em\u003e\u003c\/p\u003e\r\n\r\n\r\n\u003cp\u003e\u003cfont size=\"4\"\u003eRicha Salwan (Edited by), Richa Salwan (Author), Vivek Sharma (Edited by), Vivek Sharma (Author)\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e9780323950787, Elsevier Science\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003ePaperback, published 16 June 2023\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e206 pages\u003cbr\u003e23.5 x 19 x 1.4 cm, 0.45 kg\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\r\n\r\n\r\n\u003cp align=\"justify\"\u003e\u003cstrong\u003e\u003cfont size=\"3\"\u003e\u003cp\u003e\u003ci\u003eLaboratory Methods in Microbiology and Molecular Biology\u003c\/i\u003e describes various microbiological, biochemical, and molecular methods employed for the characterization, identification, and analysis of actinomycetes, bacteria and fungi. The book details general guidelines, expectations, and responsibilities for good lab practices and consists of chapters that covers basic microbiological, physiological, biochemical, and molecular aspects as well as in silico analysis using various bioinformatic tools. Other topics in the book include how to make solutions, microscopy and imaging of microorganisms, sero-diagnostics, and basic concepts of phylogeny, physiology, biotechnology, soil, food, and environmental microbiology while working in laboratory. \u003ci\u003eLaboratory Methods in Microbiology and Molecular Biology\u003c\/i\u003e is an informative update to current practices and future perspectives for the field of microbial biotechnology. It aims to facilitate professors, researchers, and graduate students in monitoring the precision and accuracy of the qualitative and quantitative methods in their research. \u003c\/p\u003e\u003c\/font\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e\u003cp\u003eContents List of contributors Preface Acknowledgements Introduction\u003c\/p\u003e \u003cp\u003e1. General guidelines, expectations, and responsibilities for inculcating good lab practices Richa Salwan and Vivek Sharma\u003c\/p\u003e \u003cp\u003e1. Distributing\/sharing lab responsibilities 2. Awareing students\/researchers for research ethics\/Authorship claims 3. Celebrations 4. Lab meetings 5. Reproducibility and data management 6. Scientific collection\/work life balance 7. Handling reagents\/Solutions and visiting fields 8. Laboratory safety 9. Personal safety 10. Preparing standard solutions\u003c\/p\u003e \u003cp\u003e2. Preparations of laboratory solutions Richa Salwan, Aditi Rana and Vivek Sharma\u003c\/p\u003e \u003cp\u003e1. Guidelines for preparing reagents, buffers, etc  2. General considerations for working with RNA  3. General terms and description for the preparation of reagents  4. Selection and preparation of buffer solution  References Further reading\u003c\/p\u003e \u003cp\u003e3. Basic concepts of microbiology Richa Salwan, Aditi Rana and Vivek Sharma\u003c\/p\u003e \u003cp\u003e1. Aim: preparation of culture media for the routine cultivation of bacteria  2. Aim: general techniques for the isolation and cultivation of bacteria  3. Aim: to preserve bacterial and fungal cultures by using different methods  Further reading \u003c\/p\u003e \u003cp\u003e4. Microscopy and imaging analysis of microorganisms  Richa Salwan, Aditi Rana and Vivek Sharma\u003c\/p\u003e \u003cp\u003e1. Aim: to prepare wet mount slide of the given sample 2. Aim: to observe motility of bacteria using hanging drop experiment 3. Aim: preparation of bacterial smear 4. Aim: to prepare a slide for simple staining 5. Aim: to perform negative staining of bacteria 6. Aim: to prepare a slide for Gram’s staining 7. Aim: capsule staining by anthony staining method  8. Aim: to perform capsule staining of the bacterial culture 9. Aim: to stain flagella of a bacterial culture 10. Aim: to perform spore staining of the given culture 11. Aim: perform spore staining using Dorner’s method 12. Aim: to stain the nuclear material and study the detailed morphology of the nucleus 13. Aim: to perform lactophenol cotton blue staining of the give fungal culture  14. Aim: to perform staining of a given actinomycetes culture  15. Aim: to perform acid-fast staining of a given culture  Further reading \u003c\/p\u003e \u003cp\u003e5. Characterization of microbes using molecular methods  Richa Salwan, Aditi Rana and Vivek Sharma\u003c\/p\u003e \u003cp\u003e1. Morphological characterization using Biolog  2. Microbial identification based on FAME  3. Electron microscopy  4. Molecular characterization of bacteria  Further reading \u003c\/p\u003e \u003cp\u003e6. Soil microbiology  Vivek Sharma and Richa Salwan\u003c\/p\u003e \u003cp\u003e1. Aim: Isolation of Rhizobium from the given sample  2. Aim: To isolate Actinomycetes from the given soil sample  3. Aim: Isolation of Azotobacter from soil  4. Aim: To estimate soil microbial biomass by Chloroform fumigation extraction method  5. Aim: To estimate the quantity of various enzymes in soil samples  6. Aim: To study the effect of pH on the growth of microbes  7. Aim: To study the effect of temperature on growth of microbes  8. Aim: To study the effect of salt concentration on growth of microbes  9. Aim: To perform dual culture assay or direct confrontation assay  References  Further reading \u003c\/p\u003e \u003cp\u003e7. Basic experiments in food microbiology Richa Salwan, Aditi Rana and Vivek Sharma\u003c\/p\u003e \u003cp\u003e1. Aim: Evaluation of milk quality using methylene blue reduction test (MBRT) assay  2. Aim: Isolation of lactic acid bacteria  3. Aim: To prepare yogurt under laboratory conditions  4. Aim: Preparation of bread from wheat flour  5. Aim: To prepare alcohol from grape juice under laboratory conditions  6. Aim: To prepare sauerkraut from cabbage  7. Aim: To prepare cheese under laboratory conditions  Further reading \u003c\/p\u003e \u003cp\u003e8. Basic experiments in microbial biochemistry  Richa Salwan, Aditi Rana and Vivek Sharma\u003c\/p\u003e \u003cp\u003e1. Aim: Qualitative screening of bacteria for amylase production  2. Aim: Qualitative screening of bacteria for lipase production  3. Aim: Qualitative screening of bacteria for protease production  4. Aim: Qualitative screening of bacteria for indole production  5. Aim: To perform methyl red (MR) test for detection of coliforms  6. Aim: To perform Voges-Proskauer test  7. Aim: Assessment of bacteria for utilization of sodium citrate as carbon source  8. Aim: To perform catalase test  9. Aim: Qualitative assessment of oxidase production  10. Aim: Assessment of carbohydrate fermentation  11. Aim: To perform oxidation-fermentation test  12. Aim: To perform nitrate reduction test  13. Aim: Qualitative assessment of urea hydrolysis  14. Aim: To perform triple sugar iron agar (TSIA) test  Further reading \u003c\/p\u003e \u003cp\u003e9. Analytical techniques in microbial genomics  Vivek Sharma and Richa Salwan\u003c\/p\u003e \u003cp\u003e1. Isolation of nucleic acid (DNA and RNA) from microorganisms  2. Analysis of nucleic acid (DNA and RNA) from microorganisms  References \u003c\/p\u003e \u003cp\u003e10. Analytical techniques in proteomics Richa Salwan and Vivek Sharma\u003c\/p\u003e \u003cp\u003e1. Basics of protein electrophoresis 2. SDS-PAGE  3. Consumables  4. Equipments 5. SDS-PAGE staining using Coomassie R250 or G250 6. Silver staining 7. Chemicals 8. Equipments and per-requisite 9. Method  10. Fluorescent silver staining 11. Chemicals 12. Equipments and per-requisite 13. Method Further reading\u003c\/p\u003e \u003cp\u003e11. Analytical techniques in proteomics Richa Salwan and Vivek Sharma\u003c\/p\u003e \u003cp\u003e1. Aim: Isolation and characterization of extracellular protein  2. Protein dialysis and ultrafiltration  3. Protein fractionation and desalting using gel filtration chromatography  4. Use of bioinformatics in planning a protein purification  Further reading\u003c\/p\u003e \u003cp\u003e12. Expression of recombinant proteins in prokaryotic host Richa Salwan and Vivek Sharma\u003c\/p\u003e \u003cp\u003e1. Aim: Cloning and transformation of gene of interest in expression vector 2. Background of pET vector system and expression host 3. Equipment and reagents for cloning and expression of genes 4. Protocol 5. Optimization of insert: vector molar ratios Further reading\u003c\/p\u003e \u003cp\u003e13. Sero-diagnostics in microbiology Richa Salwan and Vivek Sharma\u003c\/p\u003e \u003cp\u003e1. Enzyme-linked immunosorbent assay (ELISA) and variants of ELISA 2. Western blotting 3. Immunoprecipitation\/agglutination assay using blood sample 4. To perform ouchterlony double diffusion or passive double immunodiffusion 5. To perform radial immunodiffusion assay Further reading\u003c\/p\u003e \u003cp\u003e14. Basic tools for next-generation DNA sequencing of bacterial genome Richa Salwan and Vivek Sharma\u003c\/p\u003e \u003cp\u003e1. Background  2. Protocol  3. Reagents for illumina  4. Equipments  5. Protocol  6. Bioinformatic analyses  Further reading  Index \u003c\/p\u003e\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eSubject Areas: Microbiology [\u003ca title=\"See our other books on Microbiology\" href=\"https:\/\/freshlyprintedbooks.co.uk\/search?q=%22Microbiology%20%5Bnon-medical%5D%20%5BPSG%5D%22\"\u003enon-medical PSG\u003c\/a\u003e]\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\u003c\/font\u003e","brand":"Academic Press","offers":[{"title":"Default Title","offer_id":46651177271576,"sku":"9780323950787","price":118.76,"currency_code":"GBP","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0730\/2037\/5320\/products\/9780323950787.jpg?v=1694111069","url":"https:\/\/freshlyprintedbooks.co.uk\/products\/laboratory-methods-in-microbiology-and-molecular-biology-methods-in-molecular-microbiology-paperback-9780323950787","provider":"Freshly Printed Books","version":"1.0","type":"link"}