{"product_id":"instrumental-analysis-of-intrinsically-disordered-proteins-assessing-structure-and-conformation-hardback-9780470343418","title":"Instrumental Analysis of Intrinsically Disordered Proteins; Assessing Structure and Conformation (Hardback) 9780470343418","description":"\u003cfont face=\"Georgia\"\u003e\r\n\u003cp\u003e\u003cfont size=\"6\"\u003eInstrumental Analysis of Intrinsically Disordered Proteins\u003c\/font\u003e\u003cbr\u003e\r\n\u003cfont size=\"5\"\u003eAssessing Structure and Conformation\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\r\n\r\n\u003cp\u003e\u003cfont size=\"4\"\u003eVladimir Uversky (Author), Sonia Longhi (Author)\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e9780470343418, Wiley\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eHardback, published 14 May 2010\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e790 pages\u003cbr\u003e24.4 x 16.3 x 4.4 cm, 1.229 kg\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\r\n\u003cp align=\"justify\"\u003e\u003cem\u003e\u003cfont size=\"3\"\u003e\"With contributions from an international selection of leading researchers, this book fills an important need in a rapidly growing field. It is required reading for biochemists, biophysicists, molecular biologists, geneticists, cell biologists, physiologists, and specialists in drug design and development, proteomics, and molecular medicine with an interest in proteins and peptides.\" (Anticancer Research, 1 August 2010)\u003c\/font\u003e\u003c\/em\u003e\u003c\/p\u003e\r\n\r\n\u003cp align=\"justify\"\u003e\u003cstrong\u003e\u003cfont size=\"3\"\u003e\u003cb\u003eInstrumental techniques for analyzing intrinsically disordered proteins\u003c\/b\u003e  \u003cp\u003eThe recently recognized phenomenon of protein intrinsic disorder is gaining significant interest among researchers, especially as the number of proteins and protein domains that have been shown to be intrinsically disordered rapidly grows. The first reference to tackle this little-documented area, \u003ci\u003eInstrumental Analysis of Intrinsically Disordered Proteins: Assessing Structure and Conformation\u003c\/i\u003e provides researchers with a much-needed, comprehensive summary of recent achievements in the methods for structural characterization of intrinsically disordered proteins (IDPs).\u003c\/p\u003e \u003cp\u003eChapters discuss:\u003c\/p\u003e \u003cul\u003e \u003cli\u003e \u003cp\u003eAssessment of IDPs in the living cell\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eSpectroscopic techniques for the analysis of IDPs, including NMR and EPR spectroscopies, FTIR, circular dichroism, fluorescence spectroscopy, vibrational methods, and single-molecule analysis\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eSingle-molecule techniques applied to the study of IDPs\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eAssessment of IDP size and shape\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eTools for the analysis of IDP conformational stability\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eMass spectrometry\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eApproaches for expression and purification of IDPs\u003c\/p\u003e \u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eWith contributions from an international selection of leading researchers, \u003ci\u003eInstrumental Analysis of Intrinsically Disordered Proteins: Assessing Structure and Conformation\u003c\/i\u003e fills an important need in a rapidly growing field. It is required reading for biochemists, biophysicists, molecular biologists, geneticists, cell biologists, physiologists, and specialists in drug design and development, proteomics, and molecular medicine with an interest in proteins and peptides.\u003c\/p\u003e\u003c\/font\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003ePREFACE.  \u003cp\u003eINTRODUCTION TO THE WILEY SERIES ON PROTEIN AND PEPTIDE SCIENCE.\u003c\/p\u003e \u003cp\u003eLIST OF CONTRIBUTORS.\u003c\/p\u003e \u003cp\u003eLIST OF ABBREVIATIONS.\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePART I ASSESSING IDPs IN THE LIVING CELL.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 IDPs and Protein Degradation in The Cell\u003c\/b\u003e (\u003ci\u003eYosef Shaul, Peter Tsvetkov, and Nina Reuven\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e2 The Structural Biology of IDPs Inside Cells (\u003ci\u003ePhilipp Selenko\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePART II SPECTROSCOPIC TECHNIQUES.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Nuclear Magnetic Resonance Spectroscopy Applied to (Intrinsically) Disordered Proteins\u003c\/b\u003e (\u003ci\u003eFrans A. A. Mulder, Martin Lundqvist, and Ruud M. Scheek\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e4 Atomic-Level Characterization of Disordered Protein Ensembles Using NMR Residual Dipolar Couplings (\u003ci\u003eMartin Blackledge, Pau Bernadó, and Malene Ringkjøbing Jensen\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e5 Determining Structural Ensembles for Intrinsically Disordered Proteins (\u003ci\u003eGary W. Daughdrill\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e6 Site-Directed Spin Labeling EPR Spectroscopy (\u003ci\u003eValérie Belle,, Sabrina Rouger, Stéphanie Costanzo, Sonia Longhi, and André Fournel\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e7 The Structure of Unfolded Peptides and Proteins Explored by Vibrational Spectroscopy (\u003ci\u003eReinhard Schweitzer-Stenner, Thomas J. Measey, Andrew M. Hagarman, and Isabelle C. Dragomir\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e8 Intrinsically Disordered Proteins and Induced Folding Studied by Fourier Transform Infrared Spectroscopy (\u003ci\u003eAntonino Natalello and Silvia Maria Doglia\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e9 Genetically Engineered Polypeptides as a Model of Intrinsically Disordered Fibrillogenic Proteins: Deep UV Resonance Raman Spectroscopic Study (\u003ci\u003eNatalya I. Topilina, Vitali Sikirzhytski, Seiichiro Higashiya, Vladimir V. Ermolenkov, John T. Welch, and Igor K. Lednev\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e10 Circular Dichroism of Intrinsically Disordered Proteins (\u003ci\u003eRobert W. Woody\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e11 Fluorescence Spectroscopy of Intrinsically Disordered Proteins (\u003ci\u003eEugene A. Permyakov and Vladimir N. Uversky\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e12 Hydration of Intrinsically Disordered Proteins From Wide-Line NMR (\u003ci\u003eKálmán Tompa, Monika Bokor, and Peter Tompa\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePART III SINGLE-MOLECULE TECHNIQUES.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e13 Single-Molecule Spectroscopy of Unfolded Proteins\u003c\/b\u003e (\u003ci\u003eBenjamin Schuler\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e14 Monitoring the Conformational Equilibria of Monomeric Intrinsically Disordered Proteins by Single-Molecule Force Spectroscopy (\u003ci\u003eMassimo Sandal, Marco Brucale, and Bruno Samorì\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePART IV METHODS TO ASSESS PROTEIN SIZE AND SHAPE.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e15 Analytical Ultracentrifugation, a Useful Tool to Probe Intrinsically Disordered Proteins\u003c\/b\u003e (\u003ci\u003eFlorence Manon and Christine Ebel\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e16 Structural Insights into Intrinsically Disordered Proteins by Small-Angle X-Ray Scattering (\u003ci\u003ePau Bernadó and Dmitri I. Svergun\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e17 Dynamic and Static Light Scattering (\u003ci\u003eKlaus Gast\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e18 Analyzing Intrinsically Disordered Proteins by Size Exclusion Chromatography (\u003ci\u003eVladimir N. Uversky\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePART V CONFORMATIONAL STABILITY 545\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e19 Conformational Behavior of Intrinsically Disordered Proteins: Effects of Strong Denaturants, Temperature, PH, Counterions, and Macromolecular Crowding\u003c\/b\u003e (\u003ci\u003eVladimir N. Uversky\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e20 Detecting Disordered Regions in Proteins by Limited Proteolysis (\u003ci\u003eAngelo Fontana, Patrizia Polverino de Laureto, Barbara Spolaore, Erica Frare, and Marcello Zambonin\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePART VI MASS SPECTROMETRY.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e21 Mass Spectrometry Tools for the Investigation of Structural Disorder and Conformational Transitions in Proteins\u003c\/b\u003e (\u003ci\u003eMária Šamalíková, Carlo Santambrogio, and Rita Grandori\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePART VII EXPRESSION AND PURIFICATION OF IDPS.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e22 Recombinant Production of Intrinsically Disordered Proteins for Biophysical and Structural Characterization\u003c\/b\u003e (\u003ci\u003eDmitri Tolkatchev, Josee Plamondon, Richard Gingras, Zhengding Su, and Feng Ni\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e23 Large-Scale Identifi cation of Intrinsically Disordered Proteins (\u003ci\u003eVladimir N. Uversky, Marc S. Cortese, Peter Tompa, Veronika Csizmok, and A. Keith Dunker\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e24 Purification of Intrinsically Disordered Proteins (\u003ci\u003eAviv Paz, Tzviya Zeev-Ben-Mordehai, Joel L. Sussman, and Israel Silman\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e\u003cb\u003eINDEX.\u003c\/b\u003e\u003c\/p\u003e\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eSubject Areas: Chemistry [\u003ca title=\"See our other books on Chemistry\" href=\"https:\/\/freshlyprintedbooks.co.uk\/search?q=%22Chemistry%20%5BPN%5D%22\"\u003ePN\u003c\/a\u003e]\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\u003c\/font\u003e","brand":"Wiley","offers":[{"title":"Brand New","offer_id":52276180910360,"sku":"9780470343418","price":127.79,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0730\/2037\/5320\/files\/9780470343418.jpg?v=1781364924","url":"https:\/\/freshlyprintedbooks.co.uk\/products\/instrumental-analysis-of-intrinsically-disordered-proteins-assessing-structure-and-conformation-hardback-9780470343418","provider":"Freshly Printed Books","version":"1.0","type":"link"}