{"product_id":"insect-histology-practical-laboratory-techniques-paperback-softback-9781444336962","title":"Insect Histology; Practical Laboratory Techniques (Paperback \/ softback) 9781444336962","description":"\u003cfont face=\"Georgia\"\u003e\r\n\u003cp\u003e\u003cfont size=\"6\"\u003eInsect Histology\u003c\/font\u003e\u003cbr\u003e\r\n\u003cfont size=\"5\"\u003ePractical Laboratory Techniques\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\r\n\r\n\u003cp\u003e\u003cfont size=\"4\"\u003ePedro Barbosa (Author), Deborah Berry (Author), Christina K. Kary (Author)\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e9781444336962, Wiley\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003ePaperback \/ softback, published 21 November 2014\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e368 pages\u003cbr\u003e24.4 x 18.5 x 2.3 cm, 0.862 kg\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\r\n\u003cp align=\"justify\"\u003e\u003cem\u003e\u003cfont size=\"3\"\u003e\u003cp\u003e“A very useful book to have in your library.”  (\u003ci\u003eBritish Journal of Entomology \u0026amp; Natural History\u003c\/i\u003e, 1 March 2015)\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e\u003c\/font\u003e\u003c\/em\u003e\u003c\/p\u003e\r\n\r\n\u003cp align=\"justify\"\u003e\u003cstrong\u003e\u003cfont size=\"3\"\u003e\u003cp\u003eThis helpful guide is self-contained, holding all of the information you will need for the preparation of histological slides of all types of insect tissues. It explains the essential procedures for their preparation, providing step-by-step information on each phase, for example, on fixation, dehydrating, clearing, embedding, and staining. \u003c\/p\u003e\n\u003cp\u003eThe laboratory manual explains the cutting-edge methodology carried out today, and provides a thorough collection of both historically established and modern insect histology techniques side-by-side. It is the first book to present these various techniques appropriate to insects. \u003c\/p\u003e\n\u003cp\u003eKey issues covered include: \u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003ethe use of genetic markers in insect histology;\u003c\/li\u003e\n\u003cli\u003eimportant approaches for the preparation of tissues and organs for SEM and TEM Fluorescence;\u003c\/li\u003e\n\u003cli\u003enew immunohistochemical techniques;\u003c\/li\u003e\n\u003cli\u003ehistological problems encountered in insect tissues such as sclerotized chitin, yolk-laden eggs, chromosomes, genitalia, etc., and how to deal with them.\u003c\/li\u003e\n\u003c\/ul\u003e \u003cp\u003ePhotographs show the likely results of procedures, components of the process, and advantages of the use of particular approaches or compounds. As a comprehensive resource, this book is for students and researchers in entomology, systematics, developmental biology, insect cell biology, and morphology, wanting a clear introduction to the procedures for the histological display of insect tissues.\u003c\/p\u003e\u003c\/font\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003ePreface ix \u003cp\u003eAcknowledgements xi\u003c\/p\u003e \u003cp\u003eIntroduction xiii\u003c\/p\u003e \u003cp\u003eAbout the companion website xiv\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Problems of sclerotized chitin: Softening insect cuticle 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e1.1 Introduction 1\u003c\/p\u003e \u003cp\u003e1.2 General Methods 3\u003c\/p\u003e \u003cp\u003e1.3 Preparations of insect eggs 14\u003c\/p\u003e \u003cp\u003e1.4 Double Embedding Techniques 16\u003c\/p\u003e \u003cp\u003eReferences 19\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 Fixation 21\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e2.1 Introduction 21\u003c\/p\u003e \u003cp\u003e2.2 Aldehyde based fixatives 21\u003c\/p\u003e \u003cp\u003e2.3 Protein denaturing 30\u003c\/p\u003e \u003cp\u003e2.4 Picric acid based 33\u003c\/p\u003e \u003cp\u003e2.5 Mercuric chloride based 37\u003c\/p\u003e \u003cp\u003e2.6 SEM\/TEM 40\u003c\/p\u003e \u003cp\u003e2.7 Other 46\u003c\/p\u003e \u003cp\u003eReferences 51\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Dehydrating, clearing, and embedding 54\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e3.1 Dehydration 54\u003c\/p\u003e \u003cp\u003e3.2 Clearing 60\u003c\/p\u003e \u003cp\u003e3.3 Embedding General 65\u003c\/p\u003e \u003cp\u003e3.4 Embedding – Ester Wax 73\u003c\/p\u003e \u003cp\u003e3.5 Embedding – Methacrylate 74\u003c\/p\u003e \u003cp\u003eReferences 77\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 Staining 79\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e4.1 Single-contrast staining – Carmines 81\u003c\/p\u003e \u003cp\u003e4.2 Single contrast staining – Nuclear Stains 83\u003c\/p\u003e \u003cp\u003e4.3 Single contrast staining – General Stains 86\u003c\/p\u003e \u003cp\u003e4.4 Single contrast staining – Golgi 89\u003c\/p\u003e \u003cp\u003e4.5 Single contrast staining – Eggs 89\u003c\/p\u003e \u003cp\u003e4.6 Single contrast staining – Silver Stains 90\u003c\/p\u003e \u003cp\u003e4.7 Polychrome staining techniques – General 92\u003c\/p\u003e \u003cp\u003e4.8 Polychrome staining – Brain\/Nerve 102\u003c\/p\u003e \u003cp\u003e4.9 Polychrome staining – blood 103\u003c\/p\u003e \u003cp\u003e4.10 Single contrast procedures for chitinous material 105\u003c\/p\u003e \u003cp\u003e4.11 Polychrome staining procedures for chitinous material 106\u003c\/p\u003e \u003cp\u003e4.12 Polychrome staining for chitinous material – KOH 110\u003c\/p\u003e \u003cp\u003e4.13 Polychrome staining for chitinous material – Differential staining of Individual Organs 111\u003c\/p\u003e \u003cp\u003e4.14 Staining of specific tissues 113\u003c\/p\u003e \u003cp\u003e4.15 Two dye combinations 114\u003c\/p\u003e \u003cp\u003eReferences 117\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 Immunohistochemical techniques 119\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e5.1 Introduction 119\u003c\/p\u003e \u003cp\u003e5.2 General immunostaining techniques 127\u003c\/p\u003e \u003cp\u003e5.3 Immunolabeling of samples for Transmission Electron Microscopy (TEM) 135\u003c\/p\u003e \u003cp\u003e5.4 Proliferation assays 140\u003c\/p\u003e \u003cp\u003e5.5 Methods to detect specific proteins 142\u003c\/p\u003e \u003cp\u003eReferences 144\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Use of genetic markers in insect histology 146\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e6.1 Introduction 146\u003c\/p\u003e \u003cp\u003e6.2 Inducible genetic markers 149\u003c\/p\u003e \u003cp\u003e6.3 Mosaic gene expression 156\u003c\/p\u003e \u003cp\u003e6.4 Fluorescent markers for live imaging and kinetic microscopy 165\u003c\/p\u003e \u003cp\u003eReferences 169\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Fluorescence 171\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e7.1 Introduction 171\u003c\/p\u003e \u003cp\u003eReferences 192\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 Mounting 194\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e8.1 Introduction 194\u003c\/p\u003e \u003cp\u003eReferences 206\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9 Preparation of whole mounts 208\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e9.1 Introduction 208\u003c\/p\u003e \u003cp\u003eReferences 229\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10 Preparation of whole mounts for staining 231\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e10.1 Introduction 231\u003c\/p\u003e \u003cp\u003e10.2 Detection of NAPDHd 237\u003c\/p\u003e \u003cp\u003e10.3 SEM 238\u003c\/p\u003e \u003cp\u003e10.4 In situ hybridization 240\u003c\/p\u003e \u003cp\u003eReferences 244\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11 Preparation of genitalia, mouthparts and other body parts 246\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eReferences 256\u003c\/p\u003e \u003cp\u003e\u003cb\u003e12 Preparation of chromosomes 258\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eReferences 288\u003c\/p\u003e \u003cp\u003e\u003cb\u003e13 Preparation of other specific insect organs and tissues 290\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e13.1 Introduction 290\u003c\/p\u003e \u003cp\u003eReferences 323\u003c\/p\u003e \u003cp\u003eAppendix Dissecting fluids and saline solutions 325\u003c\/p\u003e \u003cp\u003eIndex 333\u003c\/p\u003e\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eSubject Areas: Veterinary medicine [\u003ca title=\"See our other books on Veterinary medicine\" href=\"https:\/\/freshlyprintedbooks.co.uk\/search?q=%22Veterinary%20medicine%20%5BMZ%5D%22\"\u003eMZ\u003c\/a\u003e]\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\u003c\/font\u003e","brand":"Wiley-Blackwell","offers":[{"title":"Brand New","offer_id":52501440561432,"sku":"9781444336962","price":45.59,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0730\/2037\/5320\/files\/9781444336962.jpg?v=1786239043","url":"https:\/\/freshlyprintedbooks.co.uk\/products\/insect-histology-practical-laboratory-techniques-paperback-softback-9781444336962","provider":"Freshly Printed Books","version":"1.0","type":"link"}