{"product_id":"hplc-a-practical-users-guide-multiple-component-retail-product-parts-enclosed-9780471754015","title":"HPLC; A Practical User's Guide (Multiple-component retail product, part(s) enclosed) 9780471754015","description":"\u003cfont face=\"Georgia\"\u003e\r\n\u003cp\u003e\u003cfont size=\"6\"\u003eHPLC\u003c\/font\u003e\u003cbr\u003e\r\n\u003cfont size=\"5\"\u003eA Practical User's Guide\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\r\n\r\n\u003cp\u003e\u003cfont size=\"4\"\u003eMarvin C. McMaster (Author)\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e9780471754015, Wiley\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eMultiple-component retail product, part(s) enclosed, published 12 January 2007\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e256 pages, Charts: 10 B\u0026amp;W, 0 Color; Drawings: 38 B\u0026amp;W, 0 Color; Tables: 6 B\u0026amp;W, 0 Color; Graphs: 25 B\u0026amp;W, 0 Color\u003cbr\u003e24.1 x 16.3 x 2 cm, 0.499 kg\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\r\n\r\n\r\n\u003cp align=\"justify\"\u003e\u003cstrong\u003e\u003cfont size=\"3\"\u003eThis Second Edition of the classic handbook details how to set up an HPLC system that capitalizes on the latest innovations. It covers new techniques in high-temperature, micro-flow, and ultra-fast chromatography, the linking of an HPLC to a mass spectrometer, and more. Complete appendices and supplementary material online, this guide has everything chromatographers need to know to confidently separate, identify, purify, and quantify compounds.\u003c\/font\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e\u003cp\u003ePreface xi\u003c\/p\u003e \u003cp\u003e\u003cb\u003eI HPLC Primer 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Advantages and Disadvantages of HPLC 3\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e1.1 How It Works 4\u003c\/p\u003e \u003cp\u003e1.1.1 A Separation Model of the Column 5\u003c\/p\u003e \u003cp\u003e1.1.2 Basic Hardware: A Quick, First Look 7\u003c\/p\u003e \u003cp\u003e1.1.3 Use of Solvent Gradients 8\u003c\/p\u003e \u003cp\u003e1.1.4 Ranges of Compounds 9\u003c\/p\u003e \u003cp\u003e1.2 Other Ways to Make My Separation 9\u003c\/p\u003e \u003cp\u003e1.2.1 FPLC—Fast Protein Liquid Chromatography 10\u003c\/p\u003e \u003cp\u003e1.2.2 LC—Traditional Liquid Chromatography 10\u003c\/p\u003e \u003cp\u003e1.2.3 GLC—Gas Liquid Chromatography 11\u003c\/p\u003e \u003cp\u003e1.2.4 SFC—Supercritical Fluid Chromatography 11\u003c\/p\u003e \u003cp\u003e1.2.5 TLC—Thin Layer Chromatography 12\u003c\/p\u003e \u003cp\u003e1.2.6 EP—Electrophoresis 12\u003c\/p\u003e \u003cp\u003e1.2.7 CZE—Capillary Zone Electrophoresis 13\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 Selecting an HPLC System 15\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e2.1 Characteristic Systems 16\u003c\/p\u003e \u003cp\u003e2.1.1 Finding a Fit: Detectors and Data Processing 16\u003c\/p\u003e \u003cp\u003e2.1.2 System Models: Gradient Versus Isocratic 16\u003c\/p\u003e \u003cp\u003e2.1.3 Vendor Selection 17\u003c\/p\u003e \u003cp\u003e2.1.4 Brand Names and Clones 17\u003c\/p\u003e \u003cp\u003e2.1.5 Hardware–Service–Support 18\u003c\/p\u003e \u003cp\u003e2.2 System Cost Estimates 19\u003c\/p\u003e \u003cp\u003e2.2.1 Type I System—QC Isocratic (Cost: $10–15,000) 19\u003c\/p\u003e \u003cp\u003e2.2.2 Type II System—Research Gradient (Cost: $20–25,000) 19\u003c\/p\u003e \u003cp\u003e2.2.3 Type III System—Automated Clinical (Cost: $25–35,000) 20\u003c\/p\u003e \u003cp\u003e2.2.4 Type IV System—Automated Methods (Cost: $30–50,000) 21\u003c\/p\u003e \u003cp\u003e2.3 Columns 21\u003c\/p\u003e \u003cp\u003e2.3.1 Sizes: Analytical and Preparative 21\u003c\/p\u003e \u003cp\u003e2.3.2 Separating Modes: Selecting Only What You Need 22\u003c\/p\u003e \u003cp\u003e2.3.3 Tips on Column Use 23\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Running Your Chromatograph 25\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e3.1 Set-up and Start-up 25\u003c\/p\u003e \u003cp\u003e3.1.1 Hardware Plumbing 101: Tubing and Fittings 26\u003c\/p\u003e \u003cp\u003e3.1.2 Connecting Components 28\u003c\/p\u003e \u003cp\u003e3.1.3 Solvent Clean-up 30\u003c\/p\u003e \u003cp\u003e3.1.4 Water Purity Test 33\u003c\/p\u003e \u003cp\u003e3.1.5 Start-up System Flushing 34\u003c\/p\u003e \u003cp\u003e3.1.6 Column Preparation and Equilibration 35\u003c\/p\u003e \u003cp\u003e3.2 Sample Preparation and Column Calibration 36\u003c\/p\u003e \u003cp\u003e3.2.1 Sample Clean-up 36\u003c\/p\u003e \u003cp\u003e3.2.2 Plate Counts 37\u003c\/p\u003e \u003cp\u003e3.3 Your First Chromatogram 37\u003c\/p\u003e \u003cp\u003e3.3.1 Reproducible Injection Techniques 38\u003c\/p\u003e \u003cp\u003e3.3.2 Simple Scouting for a Mobile Phase 39\u003c\/p\u003e \u003cp\u003e3.3.3 Examining the Chromatogram 40\u003c\/p\u003e \u003cp\u003e3.3.4 Basic Calculations of Results 41\u003c\/p\u003e \u003cp\u003e\u003cb\u003eII HPLC Optimization 43\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 Separation Models 45\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e4.1 Partition 45\u003c\/p\u003e \u003cp\u003e4.1.1 Separation Parameters 48\u003c\/p\u003e \u003cp\u003e4.1.2 Efficiency Factor 49\u003c\/p\u003e \u003cp\u003e4.1.3 Separation (Chemistry) Factor 53\u003c\/p\u003e \u003cp\u003e4.2 Ion Exchange Chromatography 56\u003c\/p\u003e \u003cp\u003e4.3 Size Exclusion Chromatography 57\u003c\/p\u003e \u003cp\u003e4.4 Affinity Chromatography 59\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 Column Preparation 61\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e5.1 Column Variations 61\u003c\/p\u003e \u003cp\u003e5.2 Packing Materials and Hardware 64\u003c\/p\u003e \u003cp\u003e5.3 Column Selection 66\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Column Aging, Diagnosis, and Healing 73\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e6.1 Packing Degrading—Bonded-Phase Loss 74\u003c\/p\u003e \u003cp\u003e6.2 Dissolved Packing Material—End Voids 77\u003c\/p\u003e \u003cp\u003e6.3 Bound Material 78\u003c\/p\u003e \u003cp\u003e6.4 Pressure Increases 81\u003c\/p\u003e \u003cp\u003e6.5 Column Channeling—Center-Voids 83\u003c\/p\u003e \u003cp\u003e6.6 Normal Phase, Ion Exchange, and Size Columns 84\u003c\/p\u003e \u003cp\u003e6.7 Zirconium and Polymer Columns 86\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Partition Chromatography Modifications 89\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e7.1 Reverse-Phase and Hybrid Silica 89\u003c\/p\u003e \u003cp\u003e7.1.1 Ionization Suppression 90\u003c\/p\u003e \u003cp\u003e7.1.2 Ion Pairing 91\u003c\/p\u003e \u003cp\u003e7.1.3 Organic Modifiers 92\u003c\/p\u003e \u003cp\u003e7.1.4 Chelation 92\u003c\/p\u003e \u003cp\u003e7.2 Acidic Phase Silica 93\u003c\/p\u003e \u003cp\u003e7.3 Reverse-Phase Zirconium 93\u003c\/p\u003e \u003cp\u003e7.4 Partition Mode Selection 94\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 “Nonpartition” Chromatography 95\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e8.1 Ion Exchange 96\u003c\/p\u003e \u003cp\u003e8.1.1 Cationic: Weak and Strong 96\u003c\/p\u003e \u003cp\u003e8.1.2 Anionic: Weak and Strong 97\u003c\/p\u003e \u003cp\u003e8.2 Size Exclusion 98\u003c\/p\u003e \u003cp\u003e8.2.1 Organic Soluble Samples 98\u003c\/p\u003e \u003cp\u003e8.2.2 Hydrophilic Protein Separation 99\u003c\/p\u003e \u003cp\u003e8.3 Affinity Chromatography 101\u003c\/p\u003e \u003cp\u003e8.3.1 Column Packing Modification 102\u003c\/p\u003e \u003cp\u003e8.3.2 Chelation and Optically Active Columns 103\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9 Hardware Specifics 105\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e9.1 System Protection 105\u003c\/p\u003e \u003cp\u003e9.1.1 Filters, Guard Columns, and Saturation Columns 106\u003c\/p\u003e \u003cp\u003e9.1.2 Inert Surfaces and Connections 107\u003c\/p\u003e \u003cp\u003e9.2 Pumping 108\u003c\/p\u003e \u003cp\u003e9.2.1 High- and Low-Pressure Mixing Controllers 109\u003c\/p\u003e \u003cp\u003e9.2.2 Checking Gradient Performance 112\u003c\/p\u003e \u003cp\u003e9.3 Injectors and Autosamplers 113\u003c\/p\u003e \u003cp\u003e9.4 Detectors 116\u003c\/p\u003e \u003cp\u003e9.4.1 Mass Dependent Detectors 116\u003c\/p\u003e \u003cp\u003e9.4.2 Absorptive Detectors 119\u003c\/p\u003e \u003cp\u003e9.4.3 Specific Detectors 122\u003c\/p\u003e \u003cp\u003e9.5 Fraction Collectors 123\u003c\/p\u003e \u003cp\u003e9.6 Data Collection and Processing 123\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10 Troubleshooting and Optimization 125\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e10.1 Hardware and Tools—System Pacification 125\u003c\/p\u003e \u003cp\u003e10.2 Reverse Order Diagnosis 129\u003c\/p\u003e \u003cp\u003e10.3 Introduction to Data Acquisition 132\u003c\/p\u003e \u003cp\u003e10.4 Solvent Conservation 133\u003c\/p\u003e \u003cp\u003e\u003cb\u003eIII HPLC Utilization 135\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11 Preparative Chromatography 137\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e11.1 Analytical Preparative 138\u003c\/p\u003e \u003cp\u003e11.2 Semipreparative 139\u003c\/p\u003e \u003cp\u003e11.3 “True” Preparative 139\u003c\/p\u003e \u003cp\u003e\u003cb\u003e12 Sample Preparation and Methods Development 143\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e12.1 Sample Preparation 143\u003c\/p\u003e \u003cp\u003e12.1.1 Deproteination 144\u003c\/p\u003e \u003cp\u003e12.1.2 Extraction and Concentration 145\u003c\/p\u003e \u003cp\u003e12.1.3 SFE (Cartridge Column) Preparations 145\u003c\/p\u003e \u003cp\u003e12.1.4 Extracting Encapsulated Compounds 147\u003c\/p\u003e \u003cp\u003e12.1.5 SFE Trace Enrichment and Windowing 148\u003c\/p\u003e \u003cp\u003e12.1.6 Derivatives 151\u003c\/p\u003e \u003cp\u003e12.2 Methods Development 151\u003c\/p\u003e \u003cp\u003e12.2.1 Standards Development 152\u003c\/p\u003e \u003cp\u003e12.2.2 Samples Development 154\u003c\/p\u003e \u003cp\u003e12.3 Gradient Development 156\u003c\/p\u003e \u003cp\u003e\u003cb\u003e13 Application Logics: Separations Overview 159\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e13.1 Fat-Soluble Vitamins, Steroid, and Lipids 159\u003c\/p\u003e \u003cp\u003e13.2 Water-Soluble Vitamins, Carbohydrates, and Acids 160\u003c\/p\u003e \u003cp\u003e13.3 Nucleomics 161\u003c\/p\u003e \u003cp\u003e13.4 Proteomics 162\u003c\/p\u003e \u003cp\u003e13.5 Clinical and Forensic Drug Monitoring 163\u003c\/p\u003e \u003cp\u003e13.6 Pharmaceutical Drug Development 164\u003c\/p\u003e \u003cp\u003e13.7 Environmental and Reaction Monitoring 164\u003c\/p\u003e \u003cp\u003e13.8 Application Trends 165\u003c\/p\u003e \u003cp\u003e\u003cb\u003e14 Automation 167\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e14.1 Analog-to-Digital Interfacing 167\u003c\/p\u003e \u003cp\u003e14.2 Digital Information Exchange 169\u003c\/p\u003e \u003cp\u003e14.3 HPLC System Control and Automation 169\u003c\/p\u003e \u003cp\u003e14.4 Data Collection and Interpretation 170\u003c\/p\u003e \u003cp\u003e14.4.1 Preinjection Baseline Setting 171\u003c\/p\u003e \u003cp\u003e14.4.2 Peak Detection and Integration 171\u003c\/p\u003e \u003cp\u003e14.4.3 Quantitation: Internal\/External Standards 172\u003c\/p\u003e \u003cp\u003e14.5 Automated Methods Development 172\u003c\/p\u003e \u003cp\u003e14.5.1 Automated Isocratic Development 173\u003c\/p\u003e \u003cp\u003e14.5.2 Hinge Point Gradient Development 176\u003c\/p\u003e \u003cp\u003e14.6 Data Exportation to the Real World 177\u003c\/p\u003e \u003cp\u003e14.6.1 Word Processors: .ASC, .DOC, .RTF, .WS, .WP Formats 177\u003c\/p\u003e \u003cp\u003e14.6.2 Spread Sheets: .DIF, .WK, .XLS Formats 178\u003c\/p\u003e \u003cp\u003e14.6.3 Databases: .DB2 Format 178\u003c\/p\u003e \u003cp\u003e14.6.4 Graphics: .PCX, .TIFF, .JPG Formats 178\u003c\/p\u003e \u003cp\u003e14.6.5 Chromatographic Files: Metafiles and NetCDF 178\u003c\/p\u003e \u003cp\u003e\u003cb\u003e15 Recent Advances in LC\/MS Separations 181\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e15.1 A LC\/MS Primer 181\u003c\/p\u003e \u003cp\u003e15.1.1 Quadrupole MS and Mass Selection 183\u003c\/p\u003e \u003cp\u003e15.1.2 Other Types of MS Analyzers for LC\/MS 185\u003c\/p\u003e \u003cp\u003e15.1.3 LC\/MS Interfaces 187\u003c\/p\u003e \u003cp\u003e15.1.4 LC\/MS Computer Control and Data Processing 189\u003c\/p\u003e \u003cp\u003e15.2 Microflow Chromatography 191\u003c\/p\u003e \u003cp\u003e15.3 Ultrafast HPLC Systems 192\u003c\/p\u003e \u003cp\u003e15.4 Chip HPLC Systems 192\u003c\/p\u003e \u003cp\u003e15.5 Standardized LC\/MS in Drug Design 193\u003c\/p\u003e \u003cp\u003e\u003cb\u003e16 New Directions in HPLC 195\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e16.1 Temperature-Controlled Chromatography 195\u003c\/p\u003e \u003cp\u003e16.2 Ultrafast Chromatography 196\u003c\/p\u003e \u003cp\u003e16.3 Monolith Capillary Columns 196\u003c\/p\u003e \u003cp\u003e16.4 Micro-Parallel HPLC Systems 197\u003c\/p\u003e \u003cp\u003e16.5 Two-Dimensional HPLC Systems 197\u003c\/p\u003e \u003cp\u003e16.6 The Portable LC\/MS 198\u003c\/p\u003e \u003cp\u003e\u003cb\u003eAppendices 201\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003eAppendix A Personal Separations Guide 203\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003eAppendix B FAQs for HPLC Systems and Columns 205\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003eAppendix C Tables of Solvents and Volatile Buffers 211\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003eAppendix D Glossary of HPLC Terms 213\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003eAppendix E HPLC Troubleshooting Quick Reference 221\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003eAppendix F HPLC Laboratory Experiments 227\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eLaboratory 1—System Start-up and Column Quality Control 227\u003c\/p\u003e \u003cp\u003eLaboratory 2—Sample Preparation and Methods Development 229\u003c\/p\u003e \u003cp\u003eLaboratory 3—Column and Solvent Switching and Pacification 231\u003c\/p\u003e \u003cp\u003e\u003cb\u003eAppendix G Selected Reference List 233\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eIndex 235\u003c\/p\u003e\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eSubject Areas: Chemistry [\u003ca title=\"See our other books on Chemistry\" href=\"https:\/\/freshlyprintedbooks.co.uk\/search?q=%22Chemistry%20%5BPN%5D%22\"\u003ePN\u003c\/a\u003e]\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\u003c\/font\u003e","brand":"Wiley-Interscience","offers":[{"title":"Brand New","offer_id":52298050699544,"sku":"9780471754015","price":93.95,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0730\/2037\/5320\/files\/9780471754015.jpg?v=1781732842","url":"https:\/\/freshlyprintedbooks.co.uk\/products\/hplc-a-practical-users-guide-multiple-component-retail-product-parts-enclosed-9780471754015","provider":"Freshly Printed Books","version":"1.0","type":"link"}