{"product_id":"histological-analysis-of-endocrine-disruptive-effects-in-small-laboratory-fish-hardback-9780471763581","title":"Histological Analysis of Endocrine Disruptive Effects in Small Laboratory Fish (Hardback) 9780471763581","description":"\u003cfont face=\"Georgia\"\u003e\r\n\u003cp\u003e\u003cfont size=\"6\"\u003eHistological Analysis of Endocrine Disruptive Effects in Small Laboratory Fish\u003c\/font\u003e\u003cbr\u003e\r\n\r\n\r\n\r\n\r\n\r\n\u003c\/p\u003e\n\u003cp\u003e\u003cfont size=\"4\"\u003eDaniel Dietrich (Author), Heiko O. Krieger (Author)\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e9780471763581, Wiley\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eHardback, published 30 July 2009\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e362 pages\u003cbr\u003e24.2 x 16.1 x 2.3 cm, 0.699 kg\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\r\n\r\n\r\n\u003cp align=\"justify\"\u003e\u003cstrong\u003e\u003cfont size=\"3\"\u003e\u003cul\u003e \u003cli\u003eTimely title assembling the combined knowledge of some of the leading authorities in the field of small fish reproduction - an important topic for risk assessment and registration of chemical, agricultural, and pharmaceutical compounds\u003c\/li\u003e \u003cli\u003eProvides guidance on the microscopic structure of living tissue and evaluation of the reproductive glands of small laboratory fish\u003c\/li\u003e \u003cli\u003eIncludes state-of-the-art science along with sufficient anatomical and physiological background for understanding and interpreting test results\u003c\/li\u003e \u003cli\u003eHelps standardize the interpretation of results from aquatic bioassays and field observations, which will also clarify inconsistencies in the current scientific literature\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003e\u003cb\u003eNote:\u003c\/b\u003e CD-ROM\/DVD and other supplementary materials are not included as part of eBook file.\u003c\/p\u003e\u003c\/font\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e\u003cp\u003ePreface xi\u003c\/p\u003e \u003cp\u003eAcknowledgments xiii\u003c\/p\u003e \u003cp\u003eContributing Authors xv\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Introduction 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eReferences 3\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 Fish Species of Interest 7\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e2.1 Fathead Minnow \u003ci\u003e(Pimephales promelas) \u003c\/i\u003e8\u003c\/p\u003e \u003cp\u003e2.2 Medaka \u003ci\u003e(Oryzias latipes) \u003c\/i\u003e10\u003c\/p\u003e \u003cp\u003e2.3 Zebrafish \u003ci\u003e(Danio rerio)\u003c\/i\u003e 12\u003c\/p\u003e \u003cp\u003e2.4 Other Fish Species 13\u003c\/p\u003e \u003cp\u003eReferences 15\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Sexual Determination, Differentiation, and Gonadal Development 19\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e3.1 Primordial Germ Cells in the Primordial (Primary) Gonad 22\u003c\/p\u003e \u003cp\u003e3.1.1 Differentiation and Number of PGCs 22\u003c\/p\u003e \u003cp\u003e3.1.2 Molecular Markers of PGCs 25\u003c\/p\u003e \u003cp\u003e3.2 Reproductive Strategies 26\u003c\/p\u003e \u003cp\u003e3.3 Differentiation of the Primordial Gonad into Ovary or Testis 27\u003c\/p\u003e \u003cp\u003e3.4 Gonadal Duct Formation 32\u003c\/p\u003e \u003cp\u003e3.5 Endocrinology: Influence on Gonadogenesis 40\u003c\/p\u003e \u003cp\u003e3.6 Critical Period of Sexual Differentiation in Developing Fish 45\u003c\/p\u003e \u003cp\u003e3.7 Bi-Potentiality of Germ Cells in Adult Fish 47\u003c\/p\u003e \u003cp\u003eReferences 51\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 Female Gonad Anatomy and Morphology 66\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e4.1 Gonadogenesis: Ovary 66\u003c\/p\u003e \u003cp\u003e4.1.1 Location and Gross Organization 66\u003c\/p\u003e \u003cp\u003e4.1.2 Anatomy of the Ovary 67\u003c\/p\u003e \u003cp\u003e4.2 Hypothalamic–Pituitary–Ovarian Axis 69\u003c\/p\u003e \u003cp\u003e4.3 Cellular Structure of the Ovary 69\u003c\/p\u003e \u003cp\u003e4.3.1 Germ Cells (Oogenesis) 69\u003c\/p\u003e \u003cp\u003e4.3.2 Female Somatic Supportive Tissue 78\u003c\/p\u003e \u003cp\u003e4.3.2.1 Theca Cells 78\u003c\/p\u003e \u003cp\u003e4.3.2.2 Granulosa Cells 81\u003c\/p\u003e \u003cp\u003eReferences 81\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 Male Gonad Anatomy and Morphology 88\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e5.1 Gonadogenesis: Testes 88\u003c\/p\u003e \u003cp\u003e5.1.1 Location and Gross Organization 88\u003c\/p\u003e \u003cp\u003e5.1.2 Anatomy of the Testes 89\u003c\/p\u003e \u003cp\u003e5.1.3 Germinal Epithelium 93\u003c\/p\u003e \u003cp\u003e5.1.4 Male Germ Cells (Spermatogenesis) 95\u003c\/p\u003e \u003cp\u003e5.1.5 Male Somatic Supportive Tissue 99\u003c\/p\u003e \u003cp\u003e5.1.5.1 Sertoli Cells 99\u003c\/p\u003e \u003cp\u003e5.1.5.2 Leydig Cells 103\u003c\/p\u003e \u003cp\u003e5.1.5.3 Lobule Boundary Cells 104\u003c\/p\u003e \u003cp\u003e5.1.5.4 Sex Steroid Control of Spermatogenesis 104\u003c\/p\u003e \u003cp\u003eReferences 108\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Endocrine-Disrupting Compounds 115\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e6.1 Individual Effects 117\u003c\/p\u003e \u003cp\u003e6.1.1 Inhibition of Gametogenesis 117\u003c\/p\u003e \u003cp\u003e6.1.2 Necrosis and Apoptosis (Gamete and Stromal Cells) 119\u003c\/p\u003e \u003cp\u003e6.1.3 Atretic Follicles 121\u003c\/p\u003e \u003cp\u003e6.1.4 Sertoli Cell Hypertrophy and Hyperplasia 123\u003c\/p\u003e \u003cp\u003e6.1.5 Leydig Cell Hypertrophy and Hyperplasia 129\u003c\/p\u003e \u003cp\u003e6.1.6 Fibrosis 129\u003c\/p\u003e \u003cp\u003e6.1.7 Gonadal Duct Formation 132\u003c\/p\u003e \u003cp\u003e6.2 Effects Associated with Exposure to Specific\u003c\/p\u003e \u003cp\u003eCompounds or Compound Classes 133\u003c\/p\u003e \u003cp\u003eTable 6.1 Overt Testicular Changes in Male Fish, 134\u003c\/p\u003e \u003cp\u003eTable 6.2 Testis–Ova in Male Fish, 148\u003c\/p\u003e \u003cp\u003eTable 6.3 Testicular Fibrosis in Male Fish, 161\u003c\/p\u003e \u003cp\u003eTable 6.4 Germ Cell Effects in Male Fish, 165\u003c\/p\u003e \u003cp\u003eTable 6.5 Somatic Cell Effects in Male Fish, 181\u003c\/p\u003e \u003cp\u003eTable 6.6 Testicular Inflammation and Macrophage\u003c\/p\u003e \u003cp\u003eInfiltration in Male Fish, 186\u003c\/p\u003e \u003cp\u003eTable 6.7 Overt Ovarian Changes in Female Fish, 187\u003c\/p\u003e \u003cp\u003eTable 6.8 Ovo–Testis in Female Fish, 195\u003c\/p\u003e \u003cp\u003eTable 6.9 Ovarian Fibrosis in Female Fish, 196\u003c\/p\u003e \u003cp\u003eTable 6.10 Germ Cell Effects in Female Fish, 197\u003c\/p\u003e \u003cp\u003eTable 6.11 Germ Cell Atresia in Female Fish, 206\u003c\/p\u003e \u003cp\u003eTable 6.12 Ovarian Inflammation and Macrophage Infiltration in Female Fish, 214\u003c\/p\u003e \u003cp\u003e6.2.1 Effects in Male Fish Associated with Exposure to Specific Compounds or Compound Classes 215\u003c\/p\u003e \u003cp\u003e6.2.1.1 Testis Architecture, Integrity, and Gross Appearance 215\u003c\/p\u003e \u003cp\u003e6.2.1.2 Testis–Ova 215\u003c\/p\u003e \u003cp\u003e6.2.1.3 Fibrosis 216\u003c\/p\u003e \u003cp\u003e6.2.1.4 Germ Cell Effects 217\u003c\/p\u003e \u003cp\u003e6.2.1.5 Somatic Cell Effects 219\u003c\/p\u003e \u003cp\u003e6.2.1.6 Testicular Cell Inflammation and Macrophage Infiltration 219\u003c\/p\u003e \u003cp\u003e6.2.2 Effects in Female Fish Associated with Exposure to Specific Compounds or Compound Classes 220\u003c\/p\u003e \u003cp\u003e6.2.2.1 Ovary Architecture, Integrity, and Gross Appearance 220\u003c\/p\u003e \u003cp\u003e6.2.2.2 Ovo–Testis 221\u003c\/p\u003e \u003cp\u003e6.2.2.3 Fibrosis 221\u003c\/p\u003e \u003cp\u003e6.2.2.4 Germ Cell Effects 222\u003c\/p\u003e \u003cp\u003e6.2.2.5 Germ Cell Atresia 223\u003c\/p\u003e \u003cp\u003e6.2.2.6 Germ Cell Inflammation and Macrophage Infiltration 225\u003c\/p\u003e \u003cp\u003e6.3 Population Effects 225\u003c\/p\u003e \u003cp\u003e6.3.1 Fecundity and Fertility 226\u003c\/p\u003e \u003cp\u003e6.3.2 Breeding and Reproductive Behavior of Fishes 230\u003c\/p\u003e \u003cp\u003e6.3.3 Transgenerational Effects 230\u003c\/p\u003e \u003cp\u003eReferences 232\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Determination of Effects of Exogenous Hormones and Endocrine-like Active Compounds 247\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e7.1 Histological Processing: Microdissection versus Whole-Fish Sectioning 247\u003c\/p\u003e \u003cp\u003e7.2 Optimal Tissue Preparation and Histological Techniques 250\u003c\/p\u003e \u003cp\u003e7.3 Plane of Gonad Sectioning for Optimal Organ Representation 251\u003c\/p\u003e \u003cp\u003eReferences 252\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 Evaluation of Effects in Fish Gonads 254\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e8.1 Qualitative (Semiquantitative) versus Quantitative Evaluation 254\u003c\/p\u003e \u003cp\u003e8.2 Gonadal Staging in the Testis 255\u003c\/p\u003e \u003cp\u003e8.2.1 Qualitative Staging in the Testis 255\u003c\/p\u003e \u003cp\u003e8.2.2 Quantitative Staging in the Testis 257\u003c\/p\u003e \u003cp\u003e8.3 Gonadal Staging in the Ovary 262\u003c\/p\u003e \u003cp\u003e8.3.1 Qualitative Staging in the Ovary 262\u003c\/p\u003e \u003cp\u003e8.3.2 Quantitative Staging in the Ovary 264\u003c\/p\u003e \u003cp\u003e8.4 Qualitative Assessment of Histopathological Changes 265\u003c\/p\u003e \u003cp\u003eReferences 267\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9 Experimental Design and Statistics 270\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e9.1 Basic Considerations in Experimental Design 270\u003c\/p\u003e \u003cp\u003e9.1.1 Defining the Objectives 271\u003c\/p\u003e \u003cp\u003e9.1.2 Asking Precise Questions 271\u003c\/p\u003e \u003cp\u003e9.1.3 The Principal Question: Is There an Effect or Is There Not? 272\u003c\/p\u003e \u003cp\u003e9.1.4 Basic Mechanistic Research, Risk Assessment, and the NOEC, LOEC, and ECx Question 272\u003c\/p\u003e \u003cp\u003e9.2 Variables to be Determined and Their Inherent Biological and Mathematical Characteristics 274\u003c\/p\u003e \u003cp\u003e9.2.1 Biological Characteristics 274\u003c\/p\u003e \u003cp\u003e9.2.2 Mathematical Characteristics 278\u003c\/p\u003e \u003cp\u003e9.3 Prerequisite Statistical Concepts 282\u003c\/p\u003e \u003cp\u003e9.3.1 Sample Independence: The Experimental Unit 282\u003c\/p\u003e \u003cp\u003e9.3.2 One- or Two-Sided Testing 283\u003c\/p\u003e \u003cp\u003e9.3.3 Observable Effect Size: Minimum Observable Effect 283\u003c\/p\u003e \u003cp\u003e9.3.4 Power (1  b) to Detect an Effect 284\u003c\/p\u003e \u003cp\u003e9.3.5 Replication and Pseudoreplication 286\u003c\/p\u003e \u003cp\u003e9.3.6 Minimum Significant Difference 287\u003c\/p\u003e \u003cp\u003e9.4 Statistical Tests and Testing Situations Encountered Routinely 288\u003c\/p\u003e \u003cp\u003e9.4.1 Significance Level of Multiple Comparisons: Variable-wise Significance 288\u003c\/p\u003e \u003cp\u003e9.4.2 Significance Level for Many Different Comparisons: Simultaneous Significance 288\u003c\/p\u003e \u003cp\u003e9.4.3 Multivariate Tests 289\u003c\/p\u003e \u003cp\u003e9.4.4 Parametric Versus Nonparametric Testing 289\u003c\/p\u003e \u003cp\u003eReferences 289\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10 Conclusions 295\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eReferences 297\u003c\/p\u003e \u003cp\u003e\u003cb\u003eAppendix: Fish Preparation and Microdissection of Organs 301\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eA.1 Fish Preparation 301\u003c\/p\u003e \u003cp\u003eA.2 Microdissection of Organs 302\u003c\/p\u003e \u003cp\u003eA.3 Tissue Fixation 303\u003c\/p\u003e \u003cp\u003eA.3.1 Neutral Buffered Formalin 304\u003c\/p\u003e \u003cp\u003eA.3.2 Bouin’s Fixative 304\u003c\/p\u003e \u003cp\u003eA.3.3 Lillie’s Fixative 305\u003c\/p\u003e \u003cp\u003eA.3.4 Davidson’s Fixative 305\u003c\/p\u003e \u003cp\u003eA.3.5 Dietrich’s Fixative 306\u003c\/p\u003e \u003cp\u003eA.3.6 Glutaraldehyde 306\u003c\/p\u003e \u003cp\u003eA.4 Embedding 307\u003c\/p\u003e \u003cp\u003eA.4.1 Paraffin 307\u003c\/p\u003e \u003cp\u003eA.4.2 Glycolmethacrylate (GMA) 309\u003c\/p\u003e \u003cp\u003eA.5 Tissue Sectioning 310\u003c\/p\u003e \u003cp\u003eA.6 Sample Mounting 311\u003c\/p\u003e \u003cp\u003eA.7 Tissue Slide Staining 312\u003c\/p\u003e \u003cp\u003eA.7.1 Hematoxylin and Eosin 312\u003c\/p\u003e \u003cp\u003eA.7.2 Masson’s Trichrome Stain 314\u003c\/p\u003e \u003cp\u003eA.8 Final Processing 315\u003c\/p\u003e \u003cp\u003eA.9 Examples 316\u003c\/p\u003e \u003cp\u003eA.10 Summary 317\u003c\/p\u003e \u003cp\u003eReferences 318\u003c\/p\u003e \u003cp\u003eIndex 319\u003c\/p\u003e\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eSubject Areas: Chemistry [\u003ca title=\"See our other books on Chemistry\" href=\"https:\/\/freshlyprintedbooks.co.uk\/search?q=%22Chemistry%20%5BPN%5D%22\"\u003ePN\u003c\/a\u003e]\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\u003c\/font\u003e","brand":"Wiley-Interscience","offers":[{"title":"Brand 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