{"product_id":"fundamentals-of-light-microscopy-and-electronic-imaging-hardback-9780471692140","title":"Fundamentals of Light Microscopy and Electronic Imaging (Hardback) 9780471692140","description":"\u003cfont face=\"Georgia\"\u003e\r\n\u003cp\u003e\u003cfont size=\"6\"\u003eFundamentals of Light Microscopy and Electronic Imaging\u003c\/font\u003e\u003cbr\u003e\r\n\r\n\r\n\r\n\r\n\r\n\u003c\/p\u003e\n\u003cp\u003e\u003cfont size=\"4\"\u003eDouglas B. Murphy (Author), Michael W. Davidson (Author)\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e9780471692140, Wiley\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eHardback, published 19 October 2012\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e560 pages\u003cbr\u003e25.6 x 18.5 x 3 cm, 1.338 kg\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\r\n\u003cp align=\"justify\"\u003e\u003cem\u003e\u003cfont size=\"3\"\u003e\u003cp\u003e“This should be provided to all beginning graduate students entering microscopy labs. It describes the complicated hardware of the system, while also explaining the physics principles of microscopy on a simplistic level for basic biologists. The authors achieve a perfect balance of theory and methods.”  (\u003ci\u003eDoody’s\u003c\/i\u003e, 15 November 2013)\u003c\/p\u003e \u003cp\u003e“It should be particularly useful to researchers getting started in the field of microscopy as well as seasoned professionals. Summing Up: Highly recommended. Graduate students, researchers\/faculty, and professionals\/practitioners.”  (\u003ci\u003eChoice\u003c\/i\u003e, 1 October 2013)\u003c\/p\u003e \u003cp\u003e“In summary, Fundamentals of Light Microscopy, Second Edition is a recommended starting point for the novice in microscopy and electronic imaging.”  (\u003ci\u003eJournal of Biomedical Optics\u003c\/i\u003e, 1 February 2013)\u003c\/p\u003e\u003c\/font\u003e\u003c\/em\u003e\u003c\/p\u003e\r\n\r\n\u003cp align=\"justify\"\u003e\u003cstrong\u003e\u003cfont size=\"3\"\u003e\u003cp\u003e\u003ci\u003eFundamentals of Light Microscopy and Electronic Imaging, Second Edition\u003c\/i\u003e provides a coherent introduction to the principles and applications of the integrated optical microscope system, covering both theoretical and practical considerations. It expands and updates discussions of multi-spectral imaging, intensified digital cameras, signal colocalization, and uses of objectives, and offers guidance in the selection of microscopes and electronic cameras, as well as appropriate auxiliary optical systems and fluorescent tags.\u003cbr\u003e\u003cbr\u003eThe book is divided into three sections covering optical principles in diffraction and image formation, basic modes of light microscopy, and components of modern electronic imaging systems and image processing operations. Each chapter introduces relevant theory, followed by descriptions of instrument alignment and image interpretation. This revision includes new chapters on live cell imaging, measurement of protein dynamics, deconvolution microscopy, and interference microscopy.\u003c\/p\u003e \u003cp\u003ePowerPoint slides of the figures as well as other supplementary materials for instructors are available at a companion website:\u003c\/p\u003e \u003cp\u003e\u003ca href=\"http:\/\/www.wiley.com\/go\/murphy\/lightmicroscopy\"\u003ewww.wiley.com\/go\/murphy\/lightmicroscopy\u003c\/a\u003e\u003c\/p\u003e\u003c\/font\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e\u003cp\u003ePreface xi\u003c\/p\u003e \u003cp\u003eAcknowledgments xii\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1. Fundamentals of Light Microscopy 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eOverview 1\u003c\/p\u003e \u003cp\u003eOptical Components of the Light Microscope 1\u003c\/p\u003e \u003cp\u003eAperture and Image Planes in a Focused, Adjusted Microscope 5\u003c\/p\u003e \u003cp\u003eNote: Objectives, Eyepieces, and Eyepiece Telescopes 6\u003c\/p\u003e \u003cp\u003eKoehler Illumination 9\u003c\/p\u003e \u003cp\u003eAdjusting the Microscope for Koehler Illumination 9\u003c\/p\u003e \u003cp\u003eNote: Summary of Steps for Koehler Illumination 11\u003c\/p\u003e \u003cp\u003eNote: Focusing Oil Immersion Objectives 14\u003c\/p\u003e \u003cp\u003eFixed Tube Length versus Infinity Optical Systems 15\u003c\/p\u003e \u003cp\u003ePrecautions for Handling Optical Equipment 16\u003c\/p\u003e \u003cp\u003eCare and Maintenance of the Microscope 17\u003c\/p\u003e \u003cp\u003eExercise: Calibration of Magnification 17\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2. Light and Color 21\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eOverview 21\u003c\/p\u003e \u003cp\u003eLight as a Probe of Matter 21\u003c\/p\u003e \u003cp\u003eThe Dual Particle- and Wave-Like Nature of Light 25\u003c\/p\u003e \u003cp\u003eThe Quality of Light 26\u003c\/p\u003e \u003cp\u003eProperties of Light Perceived by the Eye 27\u003c\/p\u003e \u003cp\u003ePhysical Basis for Visual Perception and Color 28\u003c\/p\u003e \u003cp\u003eAddition and Subtraction Colors 30\u003c\/p\u003e \u003cp\u003eExercise: Complementary Colors 32\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3. Illuminators, Filters, and the Isolation of Specific Wavelengths 35\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eOverview 35\u003c\/p\u003e \u003cp\u003eIlluminators and Their Spectra 35\u003c\/p\u003e \u003cp\u003eIlluminator Alignment and Bulb Replacement 41\u003c\/p\u003e \u003cp\u003eDemonstration: Spectra of Common Light Sources 41\u003c\/p\u003e \u003cp\u003eDemonstration: Aligning a 100-W Mercury Arc Lamp in an Epi-Illuminator 43\u003c\/p\u003e \u003cp\u003eFilters for Adjusting the Intensity and Wavelength of Illumination 45\u003c\/p\u003e \u003cp\u003eEffects of Light on Living Cells 50\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4. Lenses and Geometrical Optics 53\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eOverview 53\u003c\/p\u003e \u003cp\u003eReflection and Refraction of Light 53\u003c\/p\u003e \u003cp\u003eImage Formation by a Simple Lens 56\u003c\/p\u003e \u003cp\u003eNote: Real and Virtual Images 57\u003c\/p\u003e \u003cp\u003eRules of Ray Tracing for a Simple Lens 58\u003c\/p\u003e \u003cp\u003eObject–Image Math 58\u003c\/p\u003e \u003cp\u003eThe Principal Aberrations of Lenses 62\u003c\/p\u003e \u003cp\u003eDesigns and Specifications of Objectives 65\u003c\/p\u003e \u003cp\u003eCondensers 71\u003c\/p\u003e \u003cp\u003eOculars 72\u003c\/p\u003e \u003cp\u003eMicroscope Slides and Coverslips 73\u003c\/p\u003e \u003cp\u003eThe Care and Cleaning of Optics 73\u003c\/p\u003e \u003cp\u003eExercise: Constructing and Testing an Optical Bench Microscope 76\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5. Diffraction and Interference in Image Formation 79\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eOverview 79\u003c\/p\u003e \u003cp\u003eDiffraction and Interference 80\u003c\/p\u003e \u003cp\u003eThe Diffraction Image of a Point Source of Light 83\u003c\/p\u003e \u003cp\u003eThe Constancy of Optical Path Length between Object and Image 85\u003c\/p\u003e \u003cp\u003eDemonstration: Viewing the Airy Disk with a Pinhole Aperture 85\u003c\/p\u003e \u003cp\u003eEffect of Aperture Angle on Diffraction Spot Size 87\u003c\/p\u003e \u003cp\u003eDiffraction by a Grating and Calculation of Its Line Spacing, \u003ci\u003eD\u003c\/i\u003e 89\u003c\/p\u003e \u003cp\u003eDemonstration: The Diffraction Grating 93\u003c\/p\u003e \u003cp\u003eAbbé’s Theory for Image Formation in the Microscope 94\u003c\/p\u003e \u003cp\u003eA Diffraction Pattern Is Formed in the Rear Aperture of the Objective 97\u003c\/p\u003e \u003cp\u003eDemonstration: Observing the Diffraction Image in the Rear Focal Plane of a Lens 98\u003c\/p\u003e \u003cp\u003ePreservation of Coherence: Essential Requirement for Image Formation 99\u003c\/p\u003e \u003cp\u003eExercise: Diffraction by Microscope Specimens 101\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6. Diffraction and Spatial Resolution 103\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eOverview 103\u003c\/p\u003e \u003cp\u003eNumerical Aperture 103\u003c\/p\u003e \u003cp\u003eSpatial Resolution 105\u003c\/p\u003e \u003cp\u003eDepth of Field and Depth of Focus 109\u003c\/p\u003e \u003cp\u003eOptimizing the Microscope Image: A Compromise between Spatial Resolution and Contrast 109\u003c\/p\u003e \u003cp\u003eExercise: Resolution of Striae in Diatoms 112\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7. Phase Contrast Microscopy and Darkfield Microscopy 115\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eOverview 115\u003c\/p\u003e \u003cp\u003ePhase Contrast Microscopy 115\u003c\/p\u003e \u003cp\u003eThe Behavior of Waves from Phase Objects in Brightfield Microscopy 119\u003c\/p\u003e \u003cp\u003eExercise: Determination of the Intracellular Concentration of Hemoglobin in Erythrocytes by Phase Immersion Refractometry 128\u003c\/p\u003e \u003cp\u003eDarkfield Microscopy 129\u003c\/p\u003e \u003cp\u003eExercise: Darkfield Microscopy 133\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8. Properties of Polarized Light 135\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eOverview 135\u003c\/p\u003e \u003cp\u003eThe Generation of Polarized Light 135\u003c\/p\u003e \u003cp\u003eDemonstration: Producing Polarized Light with a Polaroid Filter 137\u003c\/p\u003e \u003cp\u003ePolarization by Reflection and Scattering 139\u003c\/p\u003e \u003cp\u003eVectorial Analysis of Polarized Light Using a Dichroic Filter 139\u003c\/p\u003e \u003cp\u003eDouble Refraction in Crystals 142\u003c\/p\u003e \u003cp\u003eDemonstration: Double Refraction by a Calcite Crystal 144\u003c\/p\u003e \u003cp\u003eKinds of Birefringence 145\u003c\/p\u003e \u003cp\u003ePropagation of O and E Wavefronts in a Birefringent Crystal 146\u003c\/p\u003e \u003cp\u003eBirefringence in Biological Specimens 148\u003c\/p\u003e \u003cp\u003eGeneration of Elliptically Polarized Light by Birefringent Specimens 149\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9. Polarization Microscopy 153\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eOverview 153\u003c\/p\u003e \u003cp\u003eOptics of the Polarizing Microscope 155\u003c\/p\u003e \u003cp\u003eAdjusting the Polarizing Microscope 156\u003c\/p\u003e \u003cp\u003eAppearance of Birefringent Objects in Polarized Light 157\u003c\/p\u003e \u003cp\u003ePrinciples of Action of Retardation Plates and Three Popular Compensators 158\u003c\/p\u003e \u003cp\u003eDemonstration: Making a \u003ci\u003eλ\u003c\/i\u003e-Plate from a Piece of Cellophane 162\u003c\/p\u003e \u003cp\u003eExercise: Determination of Molecular Organization in Biological Structures Using a Full Wave Plate Compensator 167\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10. Differential Interference Contrast Microscopy and Modulation Contrast Microscopy 173\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eOverview 173\u003c\/p\u003e \u003cp\u003eThe DIC Optical System 173\u003c\/p\u003e \u003cp\u003eDemonstration: The Action of a Wollaston Prism in Polarized Light 179\u003c\/p\u003e \u003cp\u003eModulation Contrast Microscopy 190\u003c\/p\u003e \u003cp\u003eExercise: DIC Microscopy 194\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11. Fluorescence Microscopy 199\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eOverview 199\u003c\/p\u003e \u003cp\u003eApplications of Fluorescence Microscopy 201\u003c\/p\u003e \u003cp\u003ePhysical Basis of Fluorescence 202\u003c\/p\u003e \u003cp\u003eProperties of Fluorescent Dyes 205\u003c\/p\u003e \u003cp\u003eDemonstration: Fluorescence of Chlorophyll and Fluorescein 206\u003c\/p\u003e \u003cp\u003eAutofluorescence of Endogenous Molecules 211\u003c\/p\u003e \u003cp\u003eDemonstration: Fluorescence of Biological Materials under UV Light 213\u003c\/p\u003e \u003cp\u003eFluorescent Dyes and Proteins in Fluorescence Microscopy 213\u003c\/p\u003e \u003cp\u003eArrangement of Filters and the Epi-Illuminator in the Fluorescence Microscope 218\u003c\/p\u003e \u003cp\u003eObjectives and Spatial Resolution in Fluorescence Microscopy 224\u003c\/p\u003e \u003cp\u003eCauses of High Fluorescence Background 225\u003c\/p\u003e \u003cp\u003eThe Problem of Bleedthrough with Multiply Stained Specimens 227\u003c\/p\u003e \u003cp\u003eQuenching, Blinking, and Photobleaching 228\u003c\/p\u003e \u003cp\u003eExamining Fluorescent Molecules in Living Cells 230\u003c\/p\u003e \u003cp\u003e\u003cb\u003e12. Fluorescence Imaging of Dynamic Molecular Processes 233\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eOverview 233\u003c\/p\u003e \u003cp\u003eModes of Dynamic Fluorescence Imaging 234\u003c\/p\u003e \u003cp\u003eFörster Resonance Energy Transfer 236\u003c\/p\u003e \u003cp\u003eApplications 244\u003c\/p\u003e \u003cp\u003eFluorescence Recovery after Photobleaching 245\u003c\/p\u003e \u003cp\u003eTIRF Microscopy: Excitation by an Evanescent Wave 252\u003c\/p\u003e \u003cp\u003eAdvanced and Emerging Dynamic Fluoresence Techniques 261\u003c\/p\u003e \u003cp\u003e\u003cb\u003e13. Confocal Laser Scanning Microscopy 265\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eOverview 265\u003c\/p\u003e \u003cp\u003eThe Optical Principle of Confocal Imaging 267\u003c\/p\u003e \u003cp\u003eDemonstration: Isolation of Focal Plane Signals with a Confocal Pinhole 271\u003c\/p\u003e \u003cp\u003eAdvantages of CLSM over Widefield Fluorescence Systems 273\u003c\/p\u003e \u003cp\u003eCriteria Defining Image Quality and the Performance of an Electronic Imaging System 275\u003c\/p\u003e \u003cp\u003eConfocal Adjustments and Their Effects on Imaging 277\u003c\/p\u003e \u003cp\u003ePhotobleaching 286\u003c\/p\u003e \u003cp\u003eGeneral Procedure for Acquiring a Confocal Image 286\u003c\/p\u003e \u003cp\u003ePerformance Check of a Confocal System 288\u003c\/p\u003e \u003cp\u003eFast (Real-Time) Imaging in Confocal Microscopy 288\u003c\/p\u003e \u003cp\u003eSpectral Analysis: A Valuable Enhancement for Confocal Imaging 295\u003c\/p\u003e \u003cp\u003eOptical Sectioning by Structured Illumination 297\u003c\/p\u003e \u003cp\u003eDeconvolution Microscopy 298\u003c\/p\u003e \u003cp\u003eExercise: Effect of Confocal Variables on Image Quality 304\u003c\/p\u003e \u003cp\u003e\u003cb\u003e14. Two-photon Excitation Fluorescence Microscopy 307\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eOverview 307\u003c\/p\u003e \u003cp\u003eThe Problem of Photon Scattering in Deep Tissue Imaging 308\u003c\/p\u003e \u003cp\u003eTwo-Photon Excitation Is a Nonlinear Process 309\u003c\/p\u003e \u003cp\u003eLocalization of Excitation 314\u003c\/p\u003e \u003cp\u003eWhy Two-Photon Imaging Works 317\u003c\/p\u003e \u003cp\u003eResolution 318\u003c\/p\u003e \u003cp\u003eEquipment 319\u003c\/p\u003e \u003cp\u003eThree-Photon Excitation 325\u003c\/p\u003e \u003cp\u003eSecond Harmonic Generation Microscopy 326\u003c\/p\u003e \u003cp\u003e\u003cb\u003e15. Superresolution Imaging 331\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eOverview 331\u003c\/p\u003e \u003cp\u003eThe RESOLFT Concept 333\u003c\/p\u003e \u003cp\u003eSingle-Molecule Localization Microscopy 334\u003c\/p\u003e \u003cp\u003eStructured Illumination Microscopy 343\u003c\/p\u003e \u003cp\u003eStimulated Emission Depletion (STED) Microscopy: Superresolution by PSF Engineering 349\u003c\/p\u003e \u003cp\u003e\u003cb\u003e16. Imaging Living Cells with the Microscope 357\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eOverview 357\u003c\/p\u003e \u003cp\u003eLabeling Strategies for Live-Cell Imaging 358\u003c\/p\u003e \u003cp\u003eControl of Illumination 361\u003c\/p\u003e \u003cp\u003eControl of Environmental Conditions 365\u003c\/p\u003e \u003cp\u003eOptics, Detectors, and Hardware 372\u003c\/p\u003e \u003cp\u003eEvaluating Live-Cell Imaging Results 384\u003c\/p\u003e \u003cp\u003eExercise: Fluorescence Microscopy of Living Tissue Culture Cells 384\u003c\/p\u003e \u003cp\u003e\u003cb\u003e17. Fundamentals of Digital Imaging 389\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eOverview 389\u003c\/p\u003e \u003cp\u003eThe Charge-Coupled Device (CCD Imager) 390\u003c\/p\u003e \u003cp\u003eCCD Designs 396\u003c\/p\u003e \u003cp\u003eNote: Interline CCD Imagers: The Design of Choice for Biomedical Imaging 398\u003c\/p\u003e \u003cp\u003eBack-Thinned Sensors 398\u003c\/p\u003e \u003cp\u003eEMCCD Cameras: High Performance Design for Greatest Sensitivity 399\u003c\/p\u003e \u003cp\u003eScientific CMOS: The Next Generation of Scientific Imagers 400\u003c\/p\u003e \u003cp\u003eCamera Variables Affecting CCD Readout and Image Quality 401\u003c\/p\u003e \u003cp\u003eSix Terms Define Imaging Performance 404\u003c\/p\u003e \u003cp\u003eAliasing 409\u003c\/p\u003e \u003cp\u003eColor Cameras 410\u003c\/p\u003e \u003cp\u003eExercise: Evaluating the Performance of a CCD Camera 411\u003c\/p\u003e \u003cp\u003e\u003cb\u003e18. Digital Image Processing 415\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eOverview 415\u003c\/p\u003e \u003cp\u003ePreliminaries: Image Display and Data Types 416\u003c\/p\u003e \u003cp\u003eHistogram Adjustment 417\u003c\/p\u003e \u003cp\u003eAdjusting Gamma (\u003ci\u003eγ\u003c\/i\u003e) to Create Exponential LUTs 421\u003c\/p\u003e \u003cp\u003eFlat-Field Correction 421\u003c\/p\u003e \u003cp\u003eImage Processing With Filters 425\u003c\/p\u003e \u003cp\u003eSignal-to-Noise Ratio 432\u003c\/p\u003e \u003cp\u003eThe Use of Color 438\u003c\/p\u003e \u003cp\u003eImages as Research Data and Requirements for Scientific Publication 442\u003c\/p\u003e \u003cp\u003eExercise: Flat-Field Correction and Determination of S\/N Ratio 448\u003c\/p\u003e \u003cp\u003eAppendix A: Answer Key to Exercises 451\u003c\/p\u003e \u003cp\u003eAppendix B: Materials for Demonstrations and Exercises 455\u003c\/p\u003e \u003cp\u003eAppendix C: Sources of Materials for Demonstrations and Exercises 463\u003c\/p\u003e \u003cp\u003eGlossary 465\u003c\/p\u003e \u003cp\u003eMicroscopy Web Resources 509\u003c\/p\u003e \u003cp\u003eRecommended Reading 521\u003c\/p\u003e \u003cp\u003eReferences 523\u003c\/p\u003e \u003cp\u003eIndex 531\u003c\/p\u003e\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eSubject Areas: Biology, life sciences [\u003ca title=\"See our other books on Biology, life sciences\" href=\"https:\/\/freshlyprintedbooks.co.uk\/search?q=%22Biology,%20life%20sciences%20%5BPS%5D%22\"\u003ePS\u003c\/a\u003e]\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\u003c\/font\u003e","brand":"Wiley-Blackwell","offers":[{"title":"Brand New","offer_id":52298035593496,"sku":"9780471692140","price":120.59,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0730\/2037\/5320\/files\/9780471692140_17092.jpg?v=1781731895","url":"https:\/\/freshlyprintedbooks.co.uk\/products\/fundamentals-of-light-microscopy-and-electronic-imaging-hardback-9780471692140","provider":"Freshly Printed Books","version":"1.0","type":"link"}