{"product_id":"functional-analysis-of-bacterial-genes-a-practical-manual-paperback-softback-9780471490081","title":"Functional Analysis of Bacterial Genes; A Practical Manual (Paperback \/ softback) 9780471490081","description":"\u003cfont face=\"Georgia\"\u003e\r\n\u003cp\u003e\u003cfont size=\"6\"\u003eFunctional Analysis of Bacterial Genes\u003c\/font\u003e\u003cbr\u003e\r\n\u003cfont size=\"5\"\u003eA Practical Manual\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\r\n\r\n\u003cp\u003e\u003cfont size=\"4\"\u003eWolfgang Schumann (Edited by), W Schumann (Author), S. Dusko Ehrlich (Edited by), Naotake Ogasawara (Edited by)\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e9780471490081, Wiley\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003ePaperback \/ softback, published 19 December 2000\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e404 pages\u003cbr\u003e24.6 x 18.4 x 2.2 cm, 0.652 kg\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\r\n\u003cp align=\"justify\"\u003e\u003cem\u003e\u003cfont size=\"3\"\u003e\"The findings are brought together in this fine book, which may serve in the future as a model for similar publications on other cell types.\" (Microbiology Today, November 2001)\u003cbr\u003e \u003cbr\u003e \"Cet ouvrage est donc une tres bonne introduction a l'analyse du genome d'une bacterie.\" (Sciences Des Aliments - French, No.6, 2001)\u003cbr\u003e \u003cbr\u003e \"a good practical manual\" (Biochemie, No.83, 2001)\u003cbr\u003e \u003cbr\u003e \"Two teams of laboratories...analyze thousands of newly discovered bacterial genes to try to discover the function of the 40-60% of them for which that is not known.\" (SciTech Book News, Vol. 25, No. 4, December 2001)\u003c\/font\u003e\u003c\/em\u003e\u003c\/p\u003e\r\n\r\n\u003cp align=\"justify\"\u003e\u003cstrong\u003e\u003cfont size=\"3\"\u003eFunctional Analysis of Bacterial Genes: A Practical Manual summarises the principles and the key methods used to analyse the function of genes in bacteria. Written by members of the Bacillus subtilis Functional Analysis Consortium, this book provides a guide to the many approaches for the systematic analysis of gene function.\u003cbr\u003e Features include:\u003cbr\u003e * In-depth discussion of the theory and application of the methods\u003cbr\u003e * A step-by-step practical guide to each method\u003cbr\u003e * Experimental examples to indicate expected results\u003cbr\u003e * Contact information for suppliers and resource centres\u003cbr\u003e Written by internationally recognised authors, Functional Analysis of Bacterial Genes: A Practical Manual will be a valuable resource to researchers and technicians using functional analysis for many bacterial species, whether in the academic departments of microbiology or genetics, the biotechnology, biomedicine or pharmaceutical industries, or other centres of genomics and bioinformatics.\u003cbr\u003e \u003cbr\u003e \"This volume, and the pioneering effort it represents, will be of interest not only to those of us who are specialists in B. subtilis but to all microbiologists and genome scientists who face the formidable task of uncovering the functions of the myriad uncharacterised genes that are emerging from large-scale sequencing.\" - Richard Losick , The Biological Laboratories, Harvard University, Cambridge, Massachusetts, USA\u003c\/font\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eList of Contributors.\u003cbr\u003e \u003cbr\u003e Foreword.\u003cbr\u003e \u003cbr\u003e Preface.\u003cbr\u003e \u003cbr\u003e Safety.\u003cbr\u003e \u003cbr\u003e Aims of the Functional Analysis Project.\u003cbr\u003e \u003cbr\u003e In silico Genome Analysis.\u003cbr\u003e \u003cbr\u003e Inactivation of Bacillus subtilis Genes Without Known Functions.\u003cbr\u003e \u003cbr\u003e Analysis of Essential Genes.\u003cbr\u003e \u003cbr\u003e Transcriptional Analysis of Large Regions of the Bacterial Genome.\u003cbr\u003e \u003cbr\u003e Useful Methods for Transcriptional Analysis.\u003cbr\u003e \u003cbr\u003e Northern Blot Analysis with non-RI Detection Systems.\u003cbr\u003e \u003cbr\u003e Micado, an Integrative Database Dedicated to the Functional Analysis of Bacillus subtilis and Microbial Genomics.\u003cbr\u003e \u003cbr\u003e Phenotype Responses and Reporter Gene Activity from the Systematic Functional Analysis of Bacillus subtilis Unknown Genes.\u003cbr\u003e \u003cbr\u003e The Contribution of the EC Consortium to the Two-dimensional Protein Index of Bacillus subtilis.\u003cbr\u003e \u003cbr\u003e Whole-Cell Fingerprinting -\u003cbr\u003e Pyrolysis Mass Spectrometry.\u003cbr\u003e \u003cbr\u003e Metabolism of Small Molecules and Inorganics.\u003cbr\u003e \u003cbr\u003e Screening for Genes Involved in Nucleotide and Nitrogen Metabolism.\u003cbr\u003e \u003cbr\u003e Screening for Growth and lacZ Reporter Gene Induction with Metal Ions and Chelators, Amino Acid Analogs, Antibiotics, Inhibitors of Oxidative Phosphorylation and Miscellaneous Stress Factors.\u003cbr\u003e \u003cbr\u003e Nucleotide Metabolism.\u003cbr\u003e \u003cbr\u003e Utilization of Small Carbon-containing Molecules by Bacillus subtilis (S. Fillinger, et al.) .\u003cbr\u003e \u003cbr\u003e Screening for Genes Involved in the Metabolism of Small Carbon-containing Molecules.\u003cbr\u003e \u003cbr\u003e Nitrogen Sources.\u003cbr\u003e \u003cbr\u003e Induction or Repression by Nitrogen Sources.\u003cbr\u003e \u003cbr\u003e Glucose Regulation in Bacillus subtilis.\u003cbr\u003e \u003cbr\u003e Catabolite Repression by Glucose.\u003cbr\u003e \u003cbr\u003e Cell Processes and Macromolecule Metabolism: a Quest for Genes Involved in Genetic Competence, and DNA Recombination and Repair.\u003cbr\u003e \u003cbr\u003e Cell Processes and Macromolecule Metabolism: Methods for the Selection of Mutants in Genetic Competence, DNA Recombination and Repair and Protein Secretion.\u003cbr\u003e \u003cbr\u003e Molecular Tools for the Identification of Mutations Affecting Protein Secretion.\u003cbr\u003e \u003cbr\u003e Screening for Mutants Defective in Secretion.\u003cbr\u003e \u003cbr\u003e Genes Affecting Motility.\u003cbr\u003e \u003cbr\u003e Screening for Motility.\u003cbr\u003e \u003cbr\u003e Differentiation of Bacillus subtilis (A. Moir, et al.) .\u003cbr\u003e \u003cbr\u003e Methods for Rapid Screening of Properties: Spore Resistance and Germination.\u003cbr\u003e \u003cbr\u003e Genes Affecting the Cell Envelope.\u003cbr\u003e \u003cbr\u003e Analysis of Cell Envelope Components.\u003cbr\u003e \u003cbr\u003e Peptidogylcan Structure and Structural Determination in Vegetative Cells of Bacillus subtilis (A. Atrih, et al.) .\u003cbr\u003e \u003cbr\u003e Cell Cycle Gene Analysis.\u003cbr\u003e \u003cbr\u003e Identification and Analysis of Cell Cycle Genes.\u003cbr\u003e \u003cbr\u003e Endospore Formation.\u003cbr\u003e \u003cbr\u003e Isolation and Characterization of Genes Affecting Sporulation or the Cell Cycle.\u003cbr\u003e \u003cbr\u003e Selection and Characterization of Genes Affecting Germination.\u003cbr\u003e \u003cbr\u003e The Oxidative Stress Response.\u003cbr\u003e \u003cbr\u003e Screening for Increased Sensitivity or Resistance and Level of lacZ Reporter Gene Expression in Response to the Oxidizing Agents Hydrogen Peroxide and Methyl Viologen.\u003cbr\u003e \u003cbr\u003e The Osmotic Shock Response.\u003cbr\u003e \u003cbr\u003e Salt Stress.\u003cbr\u003e \u003cbr\u003e Phosphate Regulation.\u003cbr\u003e \u003cbr\u003e Screening for Mutants Affected in their Response to Phosphate.\u003cbr\u003e \u003cbr\u003e The Heat Shock Response.\u003cbr\u003e \u003cbr\u003e The Cold Shock Response.\u003cbr\u003e \u003cbr\u003e pH-Inducible Genes.\u003cbr\u003e \u003cbr\u003e Analysis of the Mutant Collection for Defects in their Temperature and pH Shock Response.\u003cbr\u003e \u003cbr\u003e Screening for Growth and lacZ Reporter Gene Induction or Repression under Glucose and Phosphate Starvation and under Heat, Oxidative and Osmotic Stress.\u003cbr\u003e \u003cbr\u003e List of Bacillus subtilis Unknown Genes with a Phenotype Determined by the Functional Analysis Project.\u003cbr\u003e \u003cbr\u003e List of Bacillus subtilis Genes of Known Function.\u003cbr\u003e \u003cbr\u003e Appendix 1: List of Bacillus subtilis Mutants Constructed by the European Functional Analysis Project.\u003cbr\u003e \u003cbr\u003e Appendix 2: List of Bacillys subtilis Mutants Constructed by the Japanese Functional Analysis Project.\u003cbr\u003e \u003cbr\u003e Appendix 3: List of Selected Suppliers.\u003cbr\u003e \u003cbr\u003e Appendix 4: Glossary.\u003cbr\u003e \u003cbr\u003e Index.\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eSubject Areas: Biology, life sciences [\u003ca title=\"See our other books on Biology, life sciences\" href=\"https:\/\/freshlyprintedbooks.co.uk\/search?q=%22Biology,%20life%20sciences%20%5BPS%5D%22\"\u003ePS\u003c\/a\u003e]\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\u003c\/font\u003e","brand":"Wiley","offers":[{"title":"Brand New","offer_id":52293491523864,"sku":"9780471490081","price":200.99,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0730\/2037\/5320\/files\/9780471490081.jpg?v=1781642138","url":"https:\/\/freshlyprintedbooks.co.uk\/products\/functional-analysis-of-bacterial-genes-a-practical-manual-paperback-softback-9780471490081","provider":"Freshly Printed Books","version":"1.0","type":"link"}