{"product_id":"fed-batch-fermentation-a-practical-guide-to-scalable-recombinant-protein-production-in-escherichia-coli-hardback-9781907568923","title":"Fed-Batch Fermentation; A Practical Guide to Scalable Recombinant Protein Production in Escherichia Coli (Hardback) 9781907568923","description":"\u003cfont face=\"Georgia\"\u003e\r\n\u003cp\u003e\u003cfont size=\"6\"\u003eFed-Batch Fermentation\u003c\/font\u003e\u003cbr\u003e\r\n\u003cfont size=\"5\"\u003eA Practical Guide to Scalable Recombinant Protein Production in Escherichia Coli\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cem\u003e\u003cp\u003eFed-batch Fermentation is primarily a practical guide for recombinant protein production in E. coli, using a Fed-batch Fermentation process.\u003c\/p\u003e\u003c\/em\u003e\u003c\/p\u003e\r\n\r\n\r\n\u003cp\u003e\u003cfont size=\"4\"\u003eG G Moulton (Author)\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e9781907568923, Elsevier Science\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eHardback, published 30 September 2014\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e188 pages\u003cbr\u003e23.3 x 15.6 x 1.9 cm, 0.45 kg\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\r\n\r\n\r\n\u003cp align=\"justify\"\u003e\u003cstrong\u003e\u003cfont size=\"3\"\u003eFed-batch Fermentation is primarily a practical guide for recombinant protein production in E. coli using a Fed-batch Fermentation process. Ideal users of this guide are teaching labs and R\u0026amp;D labs that need a quick and reproducible process for recombinant protein production. It may also be used as a template for the production of recombinant protein product for use in clinical trials. The guide highlights a method whereby a medium cell density - final Ods = 30-40 (A600) - Fed-batch Fermentation process can be accomplished within a single day with minimal supervision. This process can also be done on a small (2L) scale that is scalable to 30L or more. All reagents (media, carbon source, plasmid vector and host cell) used are widely available and are relatively inexpensive. This method has been used to produce three different protein products following cGMP guidelines for Phase I clinical studies.\u003c\/font\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e\u003cul\u003e    \u003cli\u003eList of figures and tables\u003cul\u003e \u003cli\u003eFigures\u003c\/li\u003e \u003cli\u003eTables\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e \u003cli\u003eAbout the author\u003c\/li\u003e \u003cli\u003e1: Introduction to fermentation\u003cul\u003e \u003cli\u003eAbstract\u003c\/li\u003e \u003cli\u003e1.1 A brief history of early fermentation and the discovery of DNA\u003c\/li\u003e \u003cli\u003e1.2 The rise of biotechnology I\u003c\/li\u003e \u003cli\u003e1.3 The rise of biotechnology II\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e \u003cli\u003e2: Generation of a recombinant \u003ci\u003eEscherichia coli\u003c\/i\u003e expression system\u003cul\u003e \u003cli\u003eAbstract\u003c\/li\u003e \u003cli\u003e2.1 Plasmids\u003c\/li\u003e \u003cli\u003e2.2 Cloning of foreign gene into plasmid\u003c\/li\u003e \u003cli\u003e2.3 Transcription of gene into messenger RNA (mRNA)\u003c\/li\u003e \u003cli\u003e2.4 Host cell\u003c\/li\u003e \u003cli\u003e2.5 Transformation of \u003ci\u003eE. coli\u003c\/i\u003e\n\u003c\/li\u003e \u003cli\u003e2.6 Making competent cells\u003c\/li\u003e \u003cli\u003e2.7 Expression screening of transformed host cells\u003c\/li\u003e \u003cli\u003e2.8 Bacterial growth preparation\u003c\/li\u003e \u003cli\u003e2.9 Streaking and inoculating plates\u003c\/li\u003e \u003cli\u003e2.10 Generation of a working cell bank (glycerol stock)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e \u003cli\u003e3: Recombinant fed-batch fermentation using \u003ci\u003eEscherichia coli\u003c\/i\u003e\u003cul\u003e \u003cli\u003eAbstract\u003c\/li\u003e \u003cli\u003e3.1 Growth kinetics of \u003ci\u003eE. coli\u003c\/i\u003e\n\u003c\/li\u003e \u003cli\u003e3.2 Reactor kinetics\u003c\/li\u003e \u003cli\u003e3.3 The bioreactor system\u003c\/li\u003e \u003cli\u003e3.4 Set-up and performance of a 2 liter fed-batch fermentation\u003c\/li\u003e \u003cli\u003e3.5 Analysis of fed-batch fermentation\u003c\/li\u003e \u003cli\u003e3.6 Sample preparation for SDS PAGE\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e \u003cli\u003e4: \u003ci\u003eEscherichia coli\u003c\/i\u003e produced recombinant protein: Soluble versus insoluble production\u003cul\u003e \u003cli\u003eAbstract\u003c\/li\u003e \u003cli\u003e4.1 Introduction\u003c\/li\u003e \u003cli\u003e4.2 Translation from RNA into protein\u003c\/li\u003e \u003cli\u003e4.3 The protein\u003c\/li\u003e \u003cli\u003e4.4 Soluble protein expression in \u003ci\u003eE. coli\u003c\/i\u003e\n\u003c\/li\u003e \u003cli\u003e4.5 The inclusion body\u003c\/li\u003e \u003cli\u003e4.6 Isolation and solubilization of inclusion bodies\u003c\/li\u003e \u003cli\u003e4.7 Ni purification of recombinant protein product\u003c\/li\u003e \u003cli\u003e4.8 Ni purified protein\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e \u003cli\u003e5: The future of \u003ci\u003eEscherichia coli\u003c\/i\u003e recombinant fermentation\u003cul\u003e \u003cli\u003eAbstract\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e \u003cli\u003eReferences\u003c\/li\u003e \u003cli\u003eIndex\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eSubject Areas: Microbiology [\u003ca title=\"See our other books on Microbiology\" href=\"https:\/\/freshlyprintedbooks.co.uk\/search?q=%22Microbiology%20%5Bnon-medical%5D%20%5BPSG%5D%22\"\u003enon-medical PSG\u003c\/a\u003e]\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\u003c\/font\u003e","brand":"Woodhead Publishing","offers":[{"title":"Default Title","offer_id":46648825676056,"sku":"9781907568923","price":99.99,"currency_code":"GBP","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0730\/2037\/5320\/products\/9781907568923_2031cbbe-b94f-44eb-b1b4-111eeced876f.jpg?v=1696598746","url":"https:\/\/freshlyprintedbooks.co.uk\/products\/fed-batch-fermentation-a-practical-guide-to-scalable-recombinant-protein-production-in-escherichia-coli-hardback-9781907568923","provider":"Freshly Printed Books","version":"1.0","type":"link"}