{"product_id":"even-electron-mass-spectrometry-with-biomolecule-applications-hardback-9780470118023","title":"Even Electron Mass Spectrometry with Biomolecule Applications (Hardback) 9780470118023","description":"\u003cfont face=\"Georgia\"\u003e\r\n\u003cp\u003e\u003cfont size=\"6\"\u003eEven Electron Mass Spectrometry with Biomolecule Applications\u003c\/font\u003e\u003cbr\u003e\r\n\r\n\r\n\r\n\r\n\r\n\u003c\/p\u003e\n\u003cp\u003e\u003cfont size=\"4\"\u003eBryan M. Ham (Author)\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e9780470118023, Wiley\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eHardback, published 20 May 2008\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e448 pages\u003cbr\u003e24.3 x 16 x 2.7 cm, 0.735 kg\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\r\n\r\n\r\n\u003cp align=\"justify\"\u003e\u003cstrong\u003e\u003cfont size=\"3\"\u003eIn addition to the essential theoretical background and fundamental principles, this unique reference presents a detailed, step-by-step methodology for interpreting even electron mass spectrometry results. Specific chapters are devoted to: proteomics; biomolecule spectral interpretation of small molecules; biomolecule spectral interpretation of biological macromolecules; and MALDI-TOF-Postsource Decay (PSD). Chapters feature detailed examples, questions, and problems to help readers solidify their understanding of the concepts and techniques.\u003c\/font\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e\u003cp\u003ePreface xiii\u003c\/p\u003e \u003cp\u003eAcknowledgments xiv\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Introduction and Basic Definitions 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e1.1 Definition and Description of Mass Spectrometry 1\u003c\/p\u003e \u003cp\u003e1.2 Basic Design of Mass Analyzer Instrumentation 2\u003c\/p\u003e \u003cp\u003e1.3 Mass Spectrometry of Protein, Metabolite, and Lipid Biomolecules 5\u003c\/p\u003e \u003cp\u003e1.3.1 Proteomics 5\u003c\/p\u003e \u003cp\u003e1.3.2 Metabolomics 8\u003c\/p\u003e \u003cp\u003e1.3.3 Lipidomics 8\u003c\/p\u003e \u003cp\u003e1.4 Fundamental Studies of Biological Compound Interactions 11\u003c\/p\u003e \u003cp\u003e1.5 Mass-to-Charge Ratio (\u003ci\u003em\u003c\/i\u003e\/\u003ci\u003ez\u003c\/i\u003e): How the Mass Spectrometer Separates Ions 13\u003c\/p\u003e \u003cp\u003e1.6 Exact Mass versus Nominal Mass 16\u003c\/p\u003e \u003cp\u003e1.7 Mass Accuracy and Resolution 18\u003c\/p\u003e \u003cp\u003e1.8 High-Resolution Mass Measurements 20\u003c\/p\u003e \u003cp\u003e1.9 Rings Plus Double Bonds (r + db) 23\u003c\/p\u003e \u003cp\u003e1.10 Nitrogen Rule in Mass Spectrometry 24\u003c\/p\u003e \u003cp\u003e1.11 Problems 25\u003c\/p\u003e \u003cp\u003eReferences 27\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 Ionization in Mass Spectrometry 29\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e2.1 Ionization Techniques and Sources 29\u003c\/p\u003e \u003cp\u003e2.2 Electron Ionization (EI) 30\u003c\/p\u003e \u003cp\u003e2.3 Chemical Ionization (CI) 32\u003c\/p\u003e \u003cp\u003e2.3.1 Positive Chemical Ionization 34\u003c\/p\u003e \u003cp\u003e2.3.2 Negative Chemical Ionization 39\u003c\/p\u003e \u003cp\u003e2.4 Atmospheric Pressure Chemical Ionization (APCI) 40\u003c\/p\u003e \u003cp\u003e2.5 Electrospray Ionization (ESI) 42\u003c\/p\u003e \u003cp\u003e2.6 Nanoelectrospray Ionization (Nano-ESI) 6\u003c\/p\u003e \u003cp\u003e2.7 Atmospheric Pressure Photoionization (APPI) 50\u003c\/p\u003e \u003cp\u003e2.7.1 APPI Mechanism 51\u003c\/p\u003e \u003cp\u003e2.7.2 APPI VUV Lamps 52\u003c\/p\u003e \u003cp\u003e2.7.3 APPI Sources 52\u003c\/p\u003e \u003cp\u003e2.7.4 Comparison of ESI and APPI 53\u003c\/p\u003e \u003cp\u003e2.8 Matrix-Assisted Laser Desorption Ionization (MALDI) 57\u003c\/p\u003e \u003cp\u003e2.9 Fast Atom Bombardment (FAB) 64\u003c\/p\u003e \u003cp\u003e2.9.1 Application of FAB versus EI 66\u003c\/p\u003e \u003cp\u003e2.10 Problems 68\u003c\/p\u003e \u003cp\u003eReferences 69\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Mass Analyzers in Mass Spectrometry 72\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e3.1 Mass Analyzers 72\u003c\/p\u003e \u003cp\u003e3.2 Magnetic and Electric Sector Mass Analyzer 74\u003c\/p\u003e \u003cp\u003e3.3 Time-of-Flight Mass Analyzer (TOF\/MS) 79\u003c\/p\u003e \u003cp\u003e3.4 Time-of-Flight\/Time-of-Flight Mass Analyzer (TOF-TOF\/MS) 83\u003c\/p\u003e \u003cp\u003e3.5 Quadrupole Mass Filter 86\u003c\/p\u003e \u003cp\u003e3.6 Triple Quadrupole Mass Analyzer (QQQ\/MS) 91\u003c\/p\u003e \u003cp\u003e3.7 Three-Dimensional Quadrupole Ion Trap Mass Analyzer (QIT\/MS) 93\u003c\/p\u003e \u003cp\u003e3.8 Linear Quadrupole Ion Trap Mass Analyzer (LTQ\/MS) 98\u003c\/p\u003e \u003cp\u003e3.9 Quadrupole Time-of-Flight Mass Analyzer (Q-TOF\/MS) 101\u003c\/p\u003e \u003cp\u003e3.10 Fourier Transform Ion Cyclotron Resonance Mass Analyzer (FTICR\/MS) 103\u003c\/p\u003e \u003cp\u003e3.10.1 Introduction 103\u003c\/p\u003e \u003cp\u003e3.10.2 FTICR Mass Analyzer 105\u003c\/p\u003e \u003cp\u003e3.10.3 FTICR Trapped Ion Behavior 106\u003c\/p\u003e \u003cp\u003e3.10.4 Cyclotron and Magnetron Ion Motion 112\u003c\/p\u003e \u003cp\u003e3.10.5 Basic Experimental Sequence 115\u003c\/p\u003e \u003cp\u003e3.11 Linear Ion Trap Fourier Transform Mass Analyzer (LTQ-FT\/MS) 118\u003c\/p\u003e \u003cp\u003e3.12 Linear Ion Trap Orbitrap Mass Analyzer (LTQ-Orbitrap\/MS) 121\u003c\/p\u003e \u003cp\u003e3.13 Problems 134\u003c\/p\u003e \u003cp\u003eReferences 135\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 Collision and Unimolecular Reaction Rate Theory 137\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e4.1 Introduction to Collision Theory 137\u003c\/p\u003e \u003cp\u003e4.2 Noncovalent Bond Dissociation Energy 142\u003c\/p\u003e \u003cp\u003e4.3 Low-Molecular-Weight BDE Predictive Model 148\u003c\/p\u003e \u003cp\u003e4.4 Computer Modeling of BDE Values 149\u003c\/p\u003e \u003cp\u003e4.5 High-Molecular-Weight BDE Predictive Model 152\u003c\/p\u003e \u003cp\u003e4.6 Noncovalent BDE of Li\u003csup\u003e+\u003c\/sup\u003e Adduct of Monopentadecanoin 153\u003c\/p\u003e \u003cp\u003e4.7 Practice Problems 155\u003c\/p\u003e \u003cp\u003e4.7.1 Problem 1 155\u003c\/p\u003e \u003cp\u003e4.7.2 Problem 2 155\u003c\/p\u003e \u003cp\u003e4.7.3 Problem 3 157\u003c\/p\u003e \u003cp\u003e4.7.4 Problem 4 157\u003c\/p\u003e \u003cp\u003e4.7.5 Problem 5 159\u003c\/p\u003e \u003cp\u003e4.8 BDE Determination of Li\u003csup\u003e+\u003c\/sup\u003e Lipid Dimer Adducts 160\u003c\/p\u003e \u003cp\u003e4.9 Covalent Apparent Threshold Energies of Li\u003csup\u003e+\u003c\/sup\u003e Adducted Acylglycerols 164\u003c\/p\u003e \u003cp\u003e4.9.1 Apparent Threshold Energy Predictive Model 165\u003c\/p\u003e \u003cp\u003e4.9.2 Apparent Threshold Energies for Lithiated Monopentadecanoin 167\u003c\/p\u003e \u003cp\u003e4.9.3 Apparent Threshold Energies for Lithiated 1-Stearin,2-Palmitin  170\u003c\/p\u003e \u003cp\u003e4.9.4 Apparent Threshold Energies for Lithiated 1,3-Dipentadecanoin  175\u003c\/p\u003e \u003cp\u003e4.10 Computational Reaction Enthalpies and Predicted Apparent Threshold Energies 177\u003c\/p\u003e \u003cp\u003e4.11 Conclusions 182\u003c\/p\u003e \u003cp\u003eReferences 183\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 the Mass Spectrum: Odd Electron Molecular Ion versus Even Electron Precursor Ion Mass Spectra 186\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e5.1 Electron Ionization Odd Electron Processes 186\u003c\/p\u003e \u003cp\u003e5.2 Oleamide Fragmentation Pathways—Odd Electron M\u003csup\u003e+●\u003c\/sup\u003e by Gas Chromatography\/Electron Ionization–Mass Spectrometry (GC\/EI-MS) 190\u003c\/p\u003e \u003cp\u003e5.3 Oleamide Fragmentation Pathways—Even Electron [M + H]\u003csup\u003e+\u003c\/sup\u003e by Electrospray Ionization\/Ion Trap Mass Spectrometry (ESI\/IT-MS) 194\u003c\/p\u003e \u003cp\u003e5.4 Problem: Methyl Oleate EI Mass Spectrum 200\u003c\/p\u003e \u003cp\u003eReferences 200\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Product Ion Spectral Interpretation 201\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e6.1 Introduction to Product Ion Spectral Interpretation 201\u003c\/p\u003e \u003cp\u003e6.2 Structural Elucidation of 1,3-Dipentadecanoin  203\u003c\/p\u003e \u003cp\u003e6.3 Problem: Lithiated Monopentadecanoin Product Ion Spectrum 213\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Biomolecule Spectral Interpretation: Proteins 214\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e7.1 Introduction to Proteomics 214\u003c\/p\u003e \u003cp\u003e7.2 Protein Structure and Chemistry 215\u003c\/p\u003e \u003cp\u003e7.3 Bottom-Up Proteomics—Mass Spectrometry of Peptides 217\u003c\/p\u003e \u003cp\u003e7.3.1 History and Strategy 217\u003c\/p\u003e \u003cp\u003e7.3.2 Protein Identification Through Product Ion Spectra 221\u003c\/p\u003e \u003cp\u003e7.3.3 High-Energy Product Ions 226\u003c\/p\u003e \u003cp\u003e7.3.4 De Novo Sequencing 228\u003c\/p\u003e \u003cp\u003e7.3.5 Electron Capture Dissociation 230\u003c\/p\u003e \u003cp\u003e7.4 Top-Down Proteomics: Mass Spectrometry of Intact Proteins 231\u003c\/p\u003e \u003cp\u003e7.4.1 Background 231\u003c\/p\u003e \u003cp\u003e7.4.2 Gas-Phase Basicity and Protein Charging 232\u003c\/p\u003e \u003cp\u003e7.4.3 Calculation of Charge State and Molecular Weight 234\u003c\/p\u003e \u003cp\u003e7.4.4 Top-Down Protein Sequencing 236\u003c\/p\u003e \u003cp\u003e7.5 Posttranslational Modification of Proteins (PTM) 239\u003c\/p\u003e \u003cp\u003e7.5.1 Three Main Types of PTM 239\u003c\/p\u003e \u003cp\u003e7.5.2 Glycosylation of Proteins 239\u003c\/p\u003e \u003cp\u003e7.5.3 Phosphorylation of Proteins 244\u003c\/p\u003e \u003cp\u003e7.5.4 Sulfation of Proteins 262\u003c\/p\u003e \u003cp\u003e7.6 Systems Biology and Bioinformatics 272\u003c\/p\u003e \u003cp\u003e7.6.1 Biomarkers in Cancer 277\u003c\/p\u003e \u003cp\u003e7.7 Problems 279\u003c\/p\u003e \u003cp\u003eReferences 280\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 Biomolecule Spectral Interpretation—small Molecules 284\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e8.1 Introduction 284\u003c\/p\u003e \u003cp\u003e8.2 Ionization Efficiency of Lipids 285\u003c\/p\u003e \u003cp\u003e8.3 Fatty Acids 287\u003c\/p\u003e \u003cp\u003e8.3.1 Negative Ion Mode Electrospray Behavior of Fatty Acids 289\u003c\/p\u003e \u003cp\u003e8.4 Quantitative Analysis by GCEI Mass Spectrometry 295\u003c\/p\u003e \u003cp\u003e8.5 Wax Esters 300\u003c\/p\u003e \u003cp\u003e8.5.1 Oxidized Wax Esters 302\u003c\/p\u003e \u003cp\u003e8.5.2 Oxidation of Monounsaturated Wax Esters by Fenton Reaction 302\u003c\/p\u003e \u003cp\u003e8.6 Sterols 305\u003c\/p\u003e \u003cp\u003e8.6.1 Synthesis of Cholesteryl Phosphate 308\u003c\/p\u003e \u003cp\u003e8.6.2 Single-Stage and High-Resolution Mass Spectrometry 309\u003c\/p\u003e \u003cp\u003e8.6.3 Proton Nuclear Magnetic Resonance 310\u003c\/p\u003e \u003cp\u003e8.6.4 Theoretical NMR Spectroscopy 310\u003c\/p\u003e \u003cp\u003e8.6.5 Structure Elucidation 310\u003c\/p\u003e \u003cp\u003e8.7 Acylglycerols 315\u003c\/p\u003e \u003cp\u003e8.7.1 Analysis of Monopentadecanoin 316\u003c\/p\u003e \u003cp\u003e8.7.2 Analysis of 1,3-Dipentadecanoin  317\u003c\/p\u003e \u003cp\u003e8.7.3 Analysis of Triheptadecanoin 318\u003c\/p\u003e \u003cp\u003e8.8 ESIMS of Phosphorylated Lipids 319\u003c\/p\u003e \u003cp\u003e8.8.1 Electrospray Ionization Behavior of Phosphorylated Lipids 322\u003c\/p\u003e \u003cp\u003e8.8.2 Positive Ion Mode ESI of Phosphorylated Lipids 323\u003c\/p\u003e \u003cp\u003e8.8.3 Negative Ion Mode ESI of Phosphorylated Lipids 326\u003c\/p\u003e \u003cp\u003e8.9 Problems 327\u003c\/p\u003e \u003cp\u003eReferences 328\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9 Biomolecule Spectral Interpretation: Biological Macromolecules 331\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e9.1 Introduction 331\u003c\/p\u003e \u003cp\u003e9.2 Carbohydrates 332\u003c\/p\u003e \u003cp\u003e9.2.1 Ionization of Oligosaccharides 334\u003c\/p\u003e \u003cp\u003e9.2.2 Carbohydrate Fragmentation 335\u003c\/p\u003e \u003cp\u003e9.2.3 Complex Oligosaccharide Structural Elucidation 338\u003c\/p\u003e \u003cp\u003e9.3 Nucleic Acids 340\u003c\/p\u003e \u003cp\u003e9.3.1 Negative Ion Mode ESI of a Yeast 76-mer tRNA\u003csup\u003ePhe\u003c\/sup\u003e 346\u003c\/p\u003e \u003cp\u003e9.3.2 Positive Ion Mode MALDI Analysis 348\u003c\/p\u003e \u003cp\u003e9.4 Problems 353\u003c\/p\u003e \u003cp\u003eReferences 353\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10 Maldi-ToF-postsource Decay (PSD) 355\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e10.1 Introduction 355\u003c\/p\u003e \u003cp\u003e10.2 Metastable Decay 356\u003c\/p\u003e \u003cp\u003e10.3 Ion Mirror Ratio Measurement of PSD Spectra 358\u003c\/p\u003e \u003cp\u003e10.4 Postsource Decay of Phosphatidylserine 358\u003c\/p\u003e \u003cp\u003e10.4.1 Problem 10.1 364\u003c\/p\u003e \u003cp\u003e10.5 Postsource Decay of Phosphatidylcholines 365\u003c\/p\u003e \u003cp\u003e10.5.1 Problem 10.2 367\u003c\/p\u003e \u003cp\u003e10.5.2 Problem 10.3 369\u003c\/p\u003e \u003cp\u003e10.6 Postsource Decay of Phosphatidylglycerol 369\u003c\/p\u003e \u003cp\u003e10.6.1 Problem 10.4 373\u003c\/p\u003e \u003cp\u003e10.6.2 Problem 10.5 373\u003c\/p\u003e \u003cp\u003eAppendix 1 Atomic Weights and Isotopic Compositions 375\u003c\/p\u003e \u003cp\u003eAppendix 2 Solutions to Chapter Problems 383\u003c\/p\u003e \u003cp\u003eAppendix 3 Fundamental Physical Constants 401\u003c\/p\u003e \u003cp\u003eGlossary 402\u003c\/p\u003e \u003cp\u003eIndex 415\u003c\/p\u003e\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eSubject Areas: Chemistry [\u003ca title=\"See our other books on Chemistry\" href=\"https:\/\/freshlyprintedbooks.co.uk\/search?q=%22Chemistry%20%5BPN%5D%22\"\u003ePN\u003c\/a\u003e]\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\u003c\/font\u003e","brand":"Wiley-Interscience","offers":[{"title":"Brand 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