{"product_id":"digital-microscopy-hardback-9780124077614","title":"Digital Microscopy (Hardback) 9780124077614","description":"\u003cfont face=\"Georgia\"\u003e\r\n\u003cp\u003e\u003cfont size=\"6\"\u003eDigital Microscopy\u003c\/font\u003e\u003cbr\u003e\r\n\r\n\r\n\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eProvides the essential tools and methods for working with digital microscopy as a technique for quantitative biological research.\u003c\/em\u003e\u003c\/p\u003e\r\n\r\n\r\n\u003cp\u003e\u003cfont size=\"4\"\u003eGreenfield Sluder (Edited by), David E. Wolf (Edited by)\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e9780124077614\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eHardback, published 26 September 2013\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e672 pages\u003cbr\u003e23.4 x 19 x 3.6 cm, 1.38 kg\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\r\n\r\n\r\n\u003cp align=\"justify\"\u003e\u003cstrong\u003e\u003cfont size=\"3\"\u003e\u003cp\u003eThe previous edition of this book marked the shift in technology from video to digital camera use with microscope use in biological science. This new edition presents some of the optical fundamentals needed to provide a quality image to the digital camera. Specifically, it covers the fundamental geometric optics of finite- and infinity-corrected microscopes, develops the concepts of physical optics and Abbe’s theory of image formation, presents the principles of Kohler illumination, and finally reviews the fundamentals of fluorescence and fluorescence microscopy. The second group of chapters deals with digital and video fundamentals: how digital and video cameras work, how to coordinate cameras with microscopes, how to deal with digital data, the fundamentals of image processing, and low light level cameras. The third group of chapters address some specialized areas of microscopy that allow sophisticated measurements of events in living cells that are below the optical limits of resolution.\u003c\/p\u003e\u003c\/font\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eMicroscope Basics.\u003cbr\u003eThe Optics of Microscope Image Formation.\u003cbr\u003eProper Alignment of the Microscope.\u003cbr\u003eMating Cameras to Microscopes.\u003cbr\u003eFundamentals of Fluorescence and Fluorescence Microscopy.\u003cbr\u003eFluorescent Protein Applications in Microscopy.\u003cbr\u003eLive Cell Fluorescence Imaging.\u003cbr\u003eWorking with Classic Video.\u003cbr\u003ePractical Aspects of Adjusting Digital Cameras.\u003cbr\u003eCameras for Digital Microscopy.\u003cbr\u003eA High-Resolution Multimode Digital Microscope System.\u003cbr\u003eElectronic Cameras for Low-Light Microscopy.\u003cbr\u003eCamera Technologies for Low Light Imaging: Overview and Relative Advantages.\u003cbr\u003eDigital Manipulation of Brightfield and Fluorescence Images: Noise Reduction, Contrast Enhancement, and Feature Extraction.\u003cbr\u003eDigital Image Files in Light Microscopy.\u003cbr\u003eHigh-Resolution Video–Enhanced Differential Interference Contrast Light Microscopy.\u003cbr\u003eQuantitative Analysis of Digital Microscope Images.\u003cbr\u003eEvaluating Optical Aberration Using Fluorescent Microspheres: Methods, Analysis, and Corrective Actions.\u003cbr\u003eRatio Imaging: Practical Considerations for Measuring Intracellular Ca++ and pH in Living Cells.\u003cbr\u003eComputational Restoration of Fluorescence Images: Noise Reduction, Deconvolution, and Pattern Recognition.\u003cbr\u003eQuantitative Fluorescence Microscopy and Image Deconvolution.\u003cbr\u003ePractical Aspects of Quantitative Confocal Microscopy.\u003cbr\u003eTheoretical Principles and Practical Considerations for Fluorescence Resonance Energy Transfer Microscopy.\u003cbr\u003eFluorescence Lifetime Imaging Microscopy.\u003cbr\u003eFluorescence Correlation Spectroscopy: Molecular Complexing in Solution and in Living Cells.\u003cbr\u003eBreaking the Resolution Limit in Light Microscopy.\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eSubject Areas: Microscopy [\u003ca title=\"See our other books on Microscopy\" href=\"https:\/\/freshlyprintedbooks.co.uk\/search?q=%22Microscopy%20%5BPDND%5D%22\"\u003ePDND\u003c\/a\u003e], Scientific equipment, experiments \u0026amp; techniques [\u003ca title=\"See our other books on Scientific equipment, experiments \u0026amp; techniques\" href=\"https:\/\/freshlyprintedbooks.co.uk\/search?q=%22Scientific%20equipment,%20experiments%20\u0026amp;%20techniques%20%5BPDN%5D%22\"\u003ePDN\u003c\/a\u003e]\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\u003c\/font\u003e","brand":"Freshly Printed Books","offers":[{"title":"Default Title","offer_id":46649393840408,"sku":"9780124077614","price":102.69,"currency_code":"GBP","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0730\/2037\/5320\/products\/9780124077614_a286e7b2-fc52-4e66-99ba-75a29e5ad450.jpg?v=1694352976","url":"https:\/\/freshlyprintedbooks.co.uk\/products\/digital-microscopy-hardback-9780124077614","provider":"Freshly Printed Books","version":"1.0","type":"link"}