{"product_id":"coupled-bioluminescent-assays-methods-evaluations-and-applications-hardback-9780470108833","title":"Coupled Bioluminescent Assays; Methods, Evaluations, and Applications (Hardback) 9780470108833","description":"\u003cfont face=\"Georgia\"\u003e\r\n\u003cp\u003e\u003cfont size=\"6\"\u003eCoupled Bioluminescent Assays\u003c\/font\u003e\u003cbr\u003e\r\n\u003cfont size=\"5\"\u003eMethods, Evaluations, and Applications\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\r\n\r\n\u003cp\u003e\u003cfont size=\"4\"\u003eMichael J. Corey (Author)\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e9780470108833, Wiley\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eHardback, published 19 December 2008\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e328 pages\u003cbr\u003e24.1 x 16 x 2 cm, 0.569 kg\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\r\n\u003cp align=\"justify\"\u003e\u003cem\u003e\u003cfont size=\"3\"\u003e\u003cp\u003e\"The chapters of the book are well balanced.... Recommended to scientist and students who want to improve their knowledge of bioluminescent coupled assays and bioluminescence in general.\" (\u003ci\u003eAnalytical and Bioanalytical Chemistry\u003c\/i\u003e, August 2009)\u003c\/p\u003e\u003c\/font\u003e\u003c\/em\u003e\u003c\/p\u003e\r\n\r\n\u003cp align=\"justify\"\u003e\u003cstrong\u003e\u003cfont size=\"3\"\u003eThis book highlights the applications of coupled bioluminescence assay techniques to real-world problems in drug discovery, environmental and chemical analysis, and biodefense. It separates theoretical aspects from the applied sections in a clear and readable way. Coupled Bioluminescent Assays, explains the uses of CB technologies across drug discovery to analyze toxicity, drug receptors, and enzymes. It covers applications in environmental analysis and biodefense, including cytotoxicity, fertilizer and explosives analysis, and nerve agent and pesticide detection. This is the premier reference on coupled bioluminescent assays for chemists, biochemists, and molecular biologists.\u003c\/font\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e\u003cp\u003ePreface xiii\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart I Background to Coupled Bioluminescent Assays 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Introduction 3\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e1.1 Introduction to Coupled Bioluminescent Assays, 3\u003c\/p\u003e \u003cp\u003e1.2 Luminescent Technologies of the Life Sciences, 4\u003c\/p\u003e \u003cp\u003e1.3 Varieties of Fluorometric Assays, 7\u003c\/p\u003e \u003cp\u003e1.3.1 Time-Resolved Fluorescence, 8\u003c\/p\u003e \u003cp\u003e1.3.2 Fluorescence Resonance Energy Transfer, 8\u003c\/p\u003e \u003cp\u003e1.3.3 Fluorescence Polarization, 9\u003c\/p\u003e \u003cp\u003e1.4 Chemiluminescence and Bioluminescence, 10\u003c\/p\u003e \u003cp\u003e1.4.1 Chemiluminescence, 11\u003c\/p\u003e \u003cp\u003e1.4.2 Bioluminescence, 13\u003c\/p\u003e \u003cp\u003e1.5 Common Bioluminescence Systems, 14\u003c\/p\u003e \u003cp\u003e1.5.1 Firefly Luciferase, 14\u003c\/p\u003e \u003cp\u003e1.5.2 Renilla Luciferase, 16\u003c\/p\u003e \u003cp\u003e1.5.3 Bacterial Luciferases, 18\u003c\/p\u003e \u003cp\u003e1.5.4 Aequorin, 19\u003c\/p\u003e \u003cp\u003e1.5.5 Other Luciferases and Photoproteins, 20\u003c\/p\u003e \u003cp\u003e1.6 A Coupled Bioluminescent Reaction, 22\u003c\/p\u003e \u003cp\u003e1.7 Summary, 23\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 Coupled Bioluminescent Reactions in Practice 24\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e2.1 Principles of Coupled Bioluminescent Reactions, 24\u003c\/p\u003e \u003cp\u003e2.1.1 Requirements for Successful Coupled Bioluminescent Assays, 24\u003c\/p\u003e \u003cp\u003e2.2 Instrumentation and Equipment for Coupled Bioluminescent Assays, 25\u003c\/p\u003e \u003cp\u003e2.2.1 Instrument Testing, 27\u003c\/p\u003e \u003cp\u003e2.2.2 Instrument Features, 28\u003c\/p\u003e \u003cp\u003e2.2.3 Luminescent Microplates, 31\u003c\/p\u003e \u003cp\u003e2.3 Coupled Bioluminescent Assay Procedures and Precautions, 32\u003c\/p\u003e \u003cp\u003e2.3.1 Coupled Enzyme Assays, 32\u003c\/p\u003e \u003cp\u003e2.3.2 Steady-State Kinetics, 36\u003c\/p\u003e \u003cp\u003e2.3.3 Coupled Bioluminescent Reactions, 38\u003c\/p\u003e \u003cp\u003e2.3.4 Recommended Precautions for Coupled Bioluminescent Reactions, 44\u003c\/p\u003e \u003cp\u003e2.4 Data Handling for Coupled Bioluminescent Assays, 45\u003c\/p\u003e \u003cp\u003e2.4.1 Analysis of Coupled Bioluminescent Assay Results by Time-Dependent Linear Regression, 46\u003c\/p\u003e \u003cp\u003e2.4.2 Performing the Linear Regression, 49\u003c\/p\u003e \u003cp\u003e2.4.3 Outliers, 50\u003c\/p\u003e \u003cp\u003e2.4.4 Special Considerations Relating to High-Throughput Screening, 52\u003c\/p\u003e \u003cp\u003e2.5 Comparison of Coupled Bioluminescent Assays with Other Methods, 53\u003c\/p\u003e \u003cp\u003e2.5.1 Coupled Bioluminescent Assays Versus Spectrophotometric Assays, 53\u003c\/p\u003e \u003cp\u003e2.5.2 Coupled Bioluminescent Assays Versus Fluorescent Assays, 54\u003c\/p\u003e \u003cp\u003e2.5.3 Coupled Bioluminescent Assays Versus Chemiluminescent Assays, 55\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart II Biomedical Applications of Coupled Bioluminescence 57\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Coupled Bioluminescent Cytotoxicity Assays 59\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e3.1 Introduction, 59\u003c\/p\u003e \u003cp\u003e3.2 Membrane Integrity Assays, 60\u003c\/p\u003e \u003cp\u003e3.2.1 Radioactive Isotope Methods, 60\u003c\/p\u003e \u003cp\u003e3.2.2 Other Labeling Methods, 64\u003c\/p\u003e \u003cp\u003e3.2.3 General Enzyme-Release Methods, 65\u003c\/p\u003e \u003cp\u003e3.3 A Coupled Bioluminescent Assay for Enzyme Release: The G3PDH-Release Assay, 70\u003c\/p\u003e \u003cp\u003e3.3.1 Development and Features, 70\u003c\/p\u003e \u003cp\u003e3.3.2 Performance Characteristics, 75\u003c\/p\u003e \u003cp\u003e3.4 Viability Assays, 82\u003c\/p\u003e \u003cp\u003e3.4.1 ATP-Release Assay, 82\u003c\/p\u003e \u003cp\u003e3.4.2 Assays of Vital Cellular Functions, 82\u003c\/p\u003e \u003cp\u003e3.4.3 Simple Cell-Growth Assays, 83\u003c\/p\u003e \u003cp\u003e3.4.4 Total Enzyme-Release Methods, 84\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 The Role of Coupled Bioluminescent Assays in Kinase Screening and Study 85\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e4.1 The Many Roles of Kinases in Biology, 85\u003c\/p\u003e \u003cp\u003e4.2 Current Standard Kinase Assays, 86\u003c\/p\u003e \u003cp\u003e4.2.1 Fluorometric Kinase Assays, 87\u003c\/p\u003e \u003cp\u003e4.2.2 Radiolabeling Kinase Assays, 88\u003c\/p\u003e \u003cp\u003e4.2.3 Fluorometric Assays Involving Detection of ADP, 89\u003c\/p\u003e \u003cp\u003e4.3 Coupled Bioluminescent Kinase Assays, 90\u003c\/p\u003e \u003cp\u003e4.3.1 A Historical Note: The Coupled Bioluminescent Creatine Kinase Assay, 90\u003c\/p\u003e \u003cp\u003e4.3.2 ATP Depletion Kinase Assays, 90\u003c\/p\u003e \u003cp\u003e4.3.3 Reverse CB Kinase Assays, 98\u003c\/p\u003e \u003cp\u003e4.3.4 Kinases as Participants in Other CB Assays, 102\u003c\/p\u003e \u003cp\u003e4.4 Conclusions, 102\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 Coupled Bioluminescent Phosphatase Assays 103\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e5.1 Introduction, 103\u003c\/p\u003e \u003cp\u003e5.2 Phosphatases, 103\u003c\/p\u003e \u003cp\u003e5.2.1 Catalytic Properties of Phosphatases, 104\u003c\/p\u003e \u003cp\u003e5.2.2 Classification of Phosphatases, 104\u003c\/p\u003e \u003cp\u003e5.3 Contemporary Phosphatase Assay Technologies, 105\u003c\/p\u003e \u003cp\u003e5.3.1 Fluorometric Phosphatase Assays, 106\u003c\/p\u003e \u003cp\u003e5.3.2 Colorimetric Phosphatase Assays, 117\u003c\/p\u003e \u003cp\u003e5.3.3 Chemiluminescent Phosphatase Assays, 119\u003c\/p\u003e \u003cp\u003e5.3.4 Radiolabeling Phosphatase Assays, 120\u003c\/p\u003e \u003cp\u003e5.4 CB Phosphatase Assays, 122\u003c\/p\u003e \u003cp\u003e5.4.1 Assays Using Phosphorylated Luciferin, 122\u003c\/p\u003e \u003cp\u003e5.4.2 Assays Based on the GPL Reaction Series, 122\u003c\/p\u003e \u003cp\u003e5.4.3 Challenges in the Development of the GPL-Coupled Bioluminescent System for Phosphate Detection, 127\u003c\/p\u003e \u003cp\u003e5.4.4 Potential Drawbacks of GPL Phosphatase Assays, 129\u003c\/p\u003e \u003cp\u003e5.5 Conclusions, 130\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Acetylcholinesterase 131\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e6.1 Introduction, 131\u003c\/p\u003e \u003cp\u003e6.2 Established AChE Assay Methods, 133\u003c\/p\u003e \u003cp\u003e6.3 Recent Developments in AChE Assay Methods, 134\u003c\/p\u003e \u003cp\u003e6.4 Evaluation of Current AChE High-Throughput Screening Methods, 135\u003c\/p\u003e \u003cp\u003e6.5 Coupled Bioluminescent Assays of AChE Activity, 136\u003c\/p\u003e \u003cp\u003e6.6 Comparison of Coupled Bioluminescent and Other Methods of Measuring AChE Activity, 138\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Measurement of Nitric Oxide Synthase Activity by Coupled Bioluminescence 139\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e7.1 Introduction, 139\u003c\/p\u003e \u003cp\u003e7.1.1 Nitric Oxide Synthases, 139\u003c\/p\u003e \u003cp\u003e7.1.2 The Nitrate Ion in Medicine and NO Biology, 141\u003c\/p\u003e \u003cp\u003e7.1.3 NO Biology, 141\u003c\/p\u003e \u003cp\u003e7.1.4 Interrelationship Among Nitrate, Nitrite, and NO, 142\u003c\/p\u003e \u003cp\u003e7.1.5 Medical Aspects of Nitrate Biology, 143\u003c\/p\u003e \u003cp\u003e7.2 Current NOS Assays, 144\u003c\/p\u003e \u003cp\u003e7.2.1 Measuring NOS Activity by Assays for Nitrite and Nitrate, 144\u003c\/p\u003e \u003cp\u003e7.2.2 “Direct” Assays of NOS Activity, 148\u003c\/p\u003e \u003cp\u003e7.2.3 Coupled Enzymatic Assays of NOS Activity, 148\u003c\/p\u003e \u003cp\u003e7.3 Conclusions, 149\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 The Coupled Bioluminescent Pyrophosphorolysis Assay 150\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e8.1 Introduction, 150\u003c\/p\u003e \u003cp\u003e8.2 Genetic Variation in Modern Medicine, 150\u003c\/p\u003e \u003cp\u003e8.3 DNA Complementarity, 152\u003c\/p\u003e \u003cp\u003e8.3.1 DNA Hybridization, 152\u003c\/p\u003e \u003cp\u003e8.3.2 Primer Extension in SNP Analysis, 153\u003c\/p\u003e \u003cp\u003e8.3.3 Primer Degradation in SNP Analysis, 154\u003c\/p\u003e \u003cp\u003e8.4 The READIT ® Pyrophosphorolysis Assay, 156\u003c\/p\u003e \u003cp\u003e8.4.1 READIT ® : Description and Technical Aspects, 156\u003c\/p\u003e \u003cp\u003e8.4.2 Performing the READIT ® Assay, 157\u003c\/p\u003e \u003cp\u003e8.4.3 Competitive Position of READIT ® , 158\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9 Coupled Luminescent Assays of G-Protein-Coupled Receptors 160\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e9.1 Introduction to G-Protein-Coupled Receptors, 160\u003c\/p\u003e \u003cp\u003e9.1.1 Structure and Function of GPCRs, 161\u003c\/p\u003e \u003cp\u003e9.1.2 GPCRs in Medicine, 165\u003c\/p\u003e \u003cp\u003e9.2 GPCR Assay Methods, 166\u003c\/p\u003e \u003cp\u003e9.2.1 Arrestins as Markers of GPCR Activity, 166\u003c\/p\u003e \u003cp\u003e9.2.2 Calcium Quantification in GPCR Assays, 169\u003c\/p\u003e \u003cp\u003e9.2.3 Quantitative cAMP Assays of GPCR Activity, 175\u003c\/p\u003e \u003cp\u003e9.2.4 GPCR Assays Involving Inositol Triphosphate Detection, 182\u003c\/p\u003e \u003cp\u003e9.2.5 GPCR Assays Involving GTP, 183\u003c\/p\u003e \u003cp\u003e9.2.6 GPCR Assays Involving Reporter Genes, 186\u003c\/p\u003e \u003cp\u003e9.2.7 Novel GPCR Assays and Other Strategies, 189\u003c\/p\u003e \u003cp\u003e9.3 Summary, 190\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10 Coupled Bioluminescent Protease Assays 191\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e10.1 Introduction, 191\u003c\/p\u003e \u003cp\u003e10.1.1 Proteases, 191\u003c\/p\u003e \u003cp\u003e10.2 Protease Assays, 193\u003c\/p\u003e \u003cp\u003e10.2.1 Chromogenic Protease Assays, 194\u003c\/p\u003e \u003cp\u003e10.2.2 Fluorometric Protease Assays, 194\u003c\/p\u003e \u003cp\u003e10.2.3 Exotic Fluorometric Protease Assays, 197\u003c\/p\u003e \u003cp\u003e10.3 Coupled Bioluminescent Protease Assays, 198\u003c\/p\u003e \u003cp\u003e10.3.1 Protease Assays Using Luciferin Derivatives, 198\u003c\/p\u003e \u003cp\u003e10.3.2 Coupled Bioluminescent Protease Assays Employing Recombinant Luminescent Proteins, 198\u003c\/p\u003e \u003cp\u003e10.4 Summary, 199\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11 Coupled Luminescent Assays Involving Aequorin 201\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e11.1 Introduction to Aequorin, 201\u003c\/p\u003e \u003cp\u003e11.1.1 Aequorin as the First Photoprotein, 201\u003c\/p\u003e \u003cp\u003e11.1.2 The Aequorin Reaction, 202\u003c\/p\u003e \u003cp\u003e11.2 Detection of Calcium in Practice, 203\u003c\/p\u003e \u003cp\u003e11.2.1 Fluorometric Quantification of Calcium, 203\u003c\/p\u003e \u003cp\u003e11.2.2 Coupled Bioluminescent Quantification of Calcium Using Aequorin, 207\u003c\/p\u003e \u003cp\u003e11.2.3 Real-Time Imaging of Calcium Flux with Aequorin, 208\u003c\/p\u003e \u003cp\u003e11.2.4 Other Uses of Aequorin in Coupled Bioluminescent Assays, 208\u003c\/p\u003e \u003cp\u003e\u003cb\u003e12 Coupled Bioluminescent Reporter Assays 209\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e12.1 Introduction to Reporter Assays, 209\u003c\/p\u003e \u003cp\u003e12.1.1 Brief History and Development of Reporter Assays, 210\u003c\/p\u003e \u003cp\u003e12.1.2 Other Non-Luminescent Reporter Proteins, 211\u003c\/p\u003e \u003cp\u003e12.2 Luciferases as Reporters of Promoter Activities, 212\u003c\/p\u003e \u003cp\u003e12.2.1 The Modern Luminescent Reporter Assay, 213\u003c\/p\u003e \u003cp\u003e12.2.2 Multicolor Light Emission by Luciferases, 214\u003c\/p\u003e \u003cp\u003e12.2.3 Dual-Mode Reporter Assays in Practice, 214\u003c\/p\u003e \u003cp\u003e12.3 Aequorin as a Reporter Enzyme, 215\u003c\/p\u003e \u003cp\u003e12.4 Vectors for Use in Reporter Assays, 216\u003c\/p\u003e \u003cp\u003e12.4.1 Systems for Study of Specific Transcriptional Modulators, 216\u003c\/p\u003e \u003cp\u003e12.4.2 Bacterial Reporter Systems, 216\u003c\/p\u003e \u003cp\u003e12.4.3 Viral Vectors, 217\u003c\/p\u003e \u003cp\u003e12.5 Summary, 217\u003c\/p\u003e \u003cp\u003e\u003cb\u003e13 Coupled Bioluminescent Assays: Regulatory Concerns 218\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e13.1 Introduction, 218\u003c\/p\u003e \u003cp\u003e13.2 Regulatory Aspects of Assay Development, 220\u003c\/p\u003e \u003cp\u003e13.2.1 Standard Operating Procedures, 220\u003c\/p\u003e \u003cp\u003e13.2.2 Philosophy of Assay Validation, 221\u003c\/p\u003e \u003cp\u003e13.2.3 Parameters for Assay Validation, 223\u003c\/p\u003e \u003cp\u003e13.2.4 Gaining Regulatory Acceptance of CB Methods, 240\u003c\/p\u003e \u003cp\u003e13.3 Summary, 240\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart III other Applications of Coupled Bioluminescence 241\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e14 Coupled Bioluminescent Determination of Bioburden and Sterility 243\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e14.1 Introduction, 243\u003c\/p\u003e \u003cp\u003e14.2 Rapid Methods of Bioburden and Sterility Assessment, 244\u003c\/p\u003e \u003cp\u003e14.2.1 Bioburden Measurement by ATP-Release Assay, 244\u003c\/p\u003e \u003cp\u003e14.2.2 Bioburden Measurement by Protein Assay, 245\u003c\/p\u003e \u003cp\u003e14.2.3 Coupled Bioluminescent Methods of Measuring Bioburden, 245\u003c\/p\u003e \u003cp\u003e\u003cb\u003e15 Environmental Applications of Coupled Bioluminescent Assays 247\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e15.1 Introduction, 247\u003c\/p\u003e \u003cp\u003e15.2 Current Methods for Environmental Monitoring of Water Quality, 248\u003c\/p\u003e \u003cp\u003e15.3 Methods of Monitoring Stream Water and Lake Water, 249\u003c\/p\u003e \u003cp\u003e15.3.1 Monitoring of Phosphate in Freshwater, 249\u003c\/p\u003e \u003cp\u003e15.3.2 Monitoring of Nitrate in Groundwater, 250\u003c\/p\u003e \u003cp\u003e15.3.3 Monitoring of Pesticides in Freshwater, 251\u003c\/p\u003e \u003cp\u003e15.3.4 Monitoring of Bacterial Content of Freshwater, 252\u003c\/p\u003e \u003cp\u003e15.4 Methods of Monitoring Drinking Water, 254\u003c\/p\u003e \u003cp\u003e15.4.1 Current Drinking Water Reports, 254\u003c\/p\u003e \u003cp\u003e15.4.2 Biohazard Monitoring in Drinking Water, 255\u003c\/p\u003e \u003cp\u003e15.4.3 Monitoring of Chemical Hazards in Drinking Water, 257\u003c\/p\u003e \u003cp\u003eAppendix A: One-Letter Amino Acid Abbreviations 259\u003c\/p\u003e \u003cp\u003eGlossary 260\u003c\/p\u003e \u003cp\u003eBibliography 265\u003c\/p\u003e \u003cp\u003eIndex 291\u003c\/p\u003e\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eSubject Areas: Chemistry [\u003ca title=\"See our other books on Chemistry\" href=\"https:\/\/freshlyprintedbooks.co.uk\/search?q=%22Chemistry%20%5BPN%5D%22\"\u003ePN\u003c\/a\u003e]\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\u003c\/font\u003e","brand":"Wiley","offers":[{"title":"Brand New","offer_id":52257071989016,"sku":"9780470108833","price":114.99,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0730\/2037\/5320\/files\/9780470108833.jpg?v=1781276283","url":"https:\/\/freshlyprintedbooks.co.uk\/products\/coupled-bioluminescent-assays-methods-evaluations-and-applications-hardback-9780470108833","provider":"Freshly Printed Books","version":"1.0","type":"link"}