{"product_id":"cilia-structure-and-motility-hardback-9780123749734","title":"Cilia: Structure and Motility (Hardback) 9780123749734","description":"\u003cfont face=\"Georgia\"\u003e\r\n\u003cp\u003e\u003cfont size=\"6\"\u003eCilia: Structure and Motility\u003c\/font\u003e\u003cbr\u003e\r\n\r\n\r\n\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eUpdated classic and highly innovative techniques essential to the study of cilia and flagella\u003c\/em\u003e\u003c\/p\u003e\r\n\r\n\r\n\u003cp\u003e\u003cfont size=\"4\"\u003eStephen M. King (Volume editor), Gregory J Pazour (Volume editor)\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e9780123749734, Elsevier Science\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eHardback, published 21 December 2009\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e344 pages\u003cbr\u003e26 x 18.3 x 2.4 cm, 0.74 kg\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\r\n\r\n\r\n\u003cp align=\"justify\"\u003e\u003cstrong\u003e\u003cfont size=\"3\"\u003eAlong with its companion volume on intraflagellar transport, this book provides researchers with a comprehensive and up-to-date source of methods for the analysis cilia and flagella, focusing primarily on approaches that have been devised or significantly extended since the last volume of Methods in Cell Biology on this topic (volume 47, 1995). Edited by Stephen M. King and Gregory J. Pazour, the newest installment of this highly acclaimed serial will serve as an essential addition to the study of cilia and flagella.\u003c\/font\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e\u003cp\u003e1. RNAi approaches to axonemal motor function in trypanosomes (Hill) 2. Homologous recombination to knock out dynein genes in Tetrahymena (Gaertig) 3. RNAi in \u003ci\u003eChlamydomonas \u003c\/i\u003e(Cerutti) 4. Planarians as a model system for analysis of ciliary assembly\/motility (Rompolas) 5. Role of nucleotides in dynein function (Shingyoji) 6. Protein modification to probe dynein interactions + purification and identification of crosslinked dynein products (Sakato) 7. Analysis of redox-sensitive dynein components (Wakabayashi) 8. Purification of dyneins from Chlamydomonas and analysis of dynein-dynein linkers (Kamiya\/King) 9. Kinase\/phosphatase-mediated control of dynein function (Sale) 10. Central pair MT complex and associated kinesins (Mitchell) 11. Calcium regulation of axonemal function In vitro motility assays (Smith) 12. Identification and analysis of dynein regulatory complex components (Porter) 13. Isolation and analysis of radial spoke proteins (Yang) 14. Purification of dyneins from other model organisms: Ciona, sea urchin, fish (Inaba) 15. Rescue of mutant phenotypes by protein electroporation (Kamiya Lab) 16. Tubulin\/Dynein interactions (Raff) 17. Measurement of beat frequency and swimming velocity Cell model reactivation in vitro assays of dynein function (Kamiya) 18. Live imaging of ependymal cilia (Lechtreck\/Witman) 19. Immunogold labeling of axonemal components in situ + flat embedding (Geimer) 20. CryoEM approaches to axonemal organization (Nicastro) 21. CryoEM of dynein-microtubule complexes (Oda\/Kikkawa) 22. Bioinformatic approaches to dynein HC classification (Yagi) 23. Bioinformatics of non-motor dynein components (Asai) 24. Biophysical approaches to dynein mechanism (Sutoh) 25. Chlamydomonas flagellar beat analysis (Foster) 26. Biophysical measurements of motor function (step size\/force production) SAXS of axonemes (Toba\/Oiwa)\u003c\/p\u003e               \u003cp\u003eCryo-Electron Microscope Tomography to Study Axonemal\u003c\/p\u003e \u003cp\u003eOrganization\u003c\/p\u003e \u003cp\u003eDaniela Nicastro\u003c\/p\u003e \u003cp\u003e2. Electron Microscopic Imaging and Analysis of Isolated\u003c\/p\u003e \u003cp\u003eDynein Particles\u003c\/p\u003e \u003cp\u003eAnthony J. Roberts and Stan A. Burgess\u003c\/p\u003e \u003cp\u003e3. Immunogold Labeling of Flagellar Components In Situ\u003c\/p\u003e \u003cp\u003eStefan Geimer\u003c\/p\u003e \u003cp\u003e4. Scanning Electron Microscopy to Examine Cells and Organs\u003c\/p\u003e \u003cp\u003eJovenal T. SanAgustin, John A. Follit, Gregory Hendricks, and Gregory J. Pazour\u003c\/p\u003e \u003cp\u003e5. X-ray Fiber Diffraction Studies on Flagellar Axonemes\u003c\/p\u003e \u003cp\u003eKazuhiro Oiwa, Shinji Kamimura, and Hiroyuki Iwamoto\u003c\/p\u003e \u003cp\u003e6. Markers for Neuronal Cilia\u003c\/p\u003e \u003cp\u003eJacqueline S. Domire and Kirk Mykytyn\u003c\/p\u003e \u003cp\u003e7. Immunofluorescence Staining of Ciliated Respiratory Epithelial Cells\u003c\/p\u003e \u003cp\u003eHeymut Omran and Niki T. Loges\u003c\/p\u003e \u003cp\u003e8. Immunoprecipitation to Examine Protein Complexes\u003c\/p\u003e \u003cp\u003eGregory J. Pazour\u003c\/p\u003e \u003cp\u003e9. Tandem Affinity Purification of Ciliopathy-Associated Protein\u003c\/p\u003e \u003cp\u003eComplexes\u003c\/p\u003e \u003cp\u003eKarsten Boldt, Jeroen van Reeuwijk, Christian Johannes Gloeckner,\u003c\/p\u003e \u003cp\u003eMarius Ueffing, and Ronald Roepman\u003c\/p\u003e \u003cp\u003e10. Crosslinking Methods: Purification and Analysis of Crosslinked Dynein Products\u003c\/p\u003e \u003cp\u003eMiho Sakato\u003c\/p\u003e \u003cp\u003e11. Analysis of the Ciliary\/Flagellar Beating of Chlamydomonas\u003c\/p\u003e \u003cp\u003eKenneth W. Foster\u003c\/p\u003e \u003cp\u003e12. Assays of Cell and Axonemal Motility in Chlamydomonas reinhardtii\u003c\/p\u003e \u003cp\u003eRitsu Kamiya\u003c\/p\u003e \u003cp\u003e13. High-Speed Digital Imaging of Ependymal Cilia in the Murine Brain\u003c\/p\u003e \u003cp\u003eKarl-Ferdinand Lechtreck, Michael J. Sanderson, and George B. Witman\u003c\/p\u003e \u003cp\u003e14. Observation of Nodal Cilia Movement and Measurement of Nodal Flow\u003c\/p\u003e \u003cp\u003eYasushi Okada and Nobutaka Hirokawa\u003c\/p\u003e \u003cp\u003e15. Modification of Mouse Nodal Flow by Applying Artificial Flow\u003c\/p\u003e \u003cp\u003eShigenori Nonaka\u003c\/p\u003e \u003cp\u003e16. Measuring Cilium-Induced Ca2þ Increases in Cultured Renal Epithelia\u003c\/p\u003e \u003cp\u003eHelle A. Praetorius\u003c\/p\u003e\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eSubject Areas: Animal physiology [\u003ca title=\"See our other books on Animal physiology\" href=\"https:\/\/freshlyprintedbooks.co.uk\/search?q=%22Animal%20physiology%20%5BPSVD%5D%22\"\u003ePSVD\u003c\/a\u003e], Cellular biology [\u003ca title=\"See our other books on Cellular biology\" href=\"https:\/\/freshlyprintedbooks.co.uk\/search?q=%22Cellular%20biology%20%5Bcytology%5D%20%5BPSF%5D%22\"\u003ecytology PSF\u003c\/a\u003e], Physiology [\u003ca title=\"See our other books on Physiology\" href=\"https:\/\/freshlyprintedbooks.co.uk\/search?q=%22Physiology%20%5BMFG%5D%22\"\u003eMFG\u003c\/a\u003e]\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\u003c\/font\u003e","brand":"Academic Press","offers":[{"title":"Default Title","offer_id":46649304482072,"sku":"9780123749734","price":83.19,"currency_code":"GBP","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0730\/2037\/5320\/products\/9780123749734_6ccd45d0-3c67-4f8f-ae8c-35c4f96f1d4e.jpg?v=1695013972","url":"https:\/\/freshlyprintedbooks.co.uk\/products\/cilia-structure-and-motility-hardback-9780123749734","provider":"Freshly Printed Books","version":"1.0","type":"link"}