{"product_id":"cell-biology-assays-essential-methods-paperback-9780123751522","title":"Cell Biology Assays; Essential Methods (Paperback \/ softback) 9780123751522","description":"\u003cfont face=\"Georgia\"\u003e\r\n\u003cp\u003e\u003cfont size=\"6\"\u003eCell Biology Assays\u003c\/font\u003e\u003cbr\u003e\r\n\u003cfont size=\"5\"\u003eEssential Methods\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cem\u003eComprehensive guide to essential methods in cell biology\u003c\/em\u003e\u003c\/p\u003e\r\n\r\n\r\n\u003cp\u003e\u003cfont size=\"4\"\u003eJulio E. Celis (Editor-in-chief), Fanny Jaulin (Edited by), Cedric Espenel (Edited by)\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e9780123751522, Elsevier Science\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003ePaperback \/ softback, published 17 November 2009\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e298 pages\u003cbr\u003e23.4 x 19 x 2 cm, 0.63 kg\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\r\n\r\n\r\n\u003cp align=\"justify\"\u003e\u003cstrong\u003e\u003cfont size=\"3\"\u003e\u003cp\u003eThis text provides comprehensive protocols essential methods across cell biology. The techniques in this text are presented in a friendly step-by-step fashion, providing useful tips and potential pitfalls while enabling researchers at all stages to embark on basic problems using a variety of technologies and model systems.\u003c\/p\u003e\u003c\/font\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e\u003col\u003e \u003cli\u003ePermeabilized Epithelial Cells to Study Exocytic Membrane Transport\u003c\/li\u003e \u003cli\u003eStudying Excit and Surface Delivery of Post-Golgi Transport Intermediates Using in vitro and Live-Cell Microscopy-Based Approaches\u003c\/li\u003e \u003cli\u003eAssays Measuring Membrane Transport in the Endocytic Pathway\u003c\/li\u003e \u003cli\u003eMicrosome-Based Assay for Analysis of Endoplasmic Reticulum to Golgi Transport In Mammalian Cells\u003c\/li\u003e \u003cli\u003eCotranslational Translocation of Proteins into Microsomes Derived from the Rough Endoplasmic Reticulum of Mammalian Cells\u003c\/li\u003e \u003cli\u003eUse of Permeabilsed Mast Cells to Analyze Regulated Exocytosis\u003c\/li\u003e \u003cli\u003eSyringe Loading: A Method for Assessing Plasma Membrane Function as a Reflection of Mechanically Induced Cell Loading\u003c\/li\u003e \u003cli\u003eCell Surface Biotinylation and Other Techniques for Determination of Surface Polarity of Epithelial Monolayers\u003c\/li\u003e \u003cli\u003eProtein Translocation into Mitochondria\u003c\/li\u003e \u003cli\u003ePolarographic Assays of Mitochrondrial Functions\u003c\/li\u003e \u003cli\u003eAnalysis of Nuclear Protein Import and Export in Digitonin-Permeabilized Cells\u003c\/li\u003e \u003cli\u003eHeterokaryons: An Assay for Nucleocytoplasmic Shuttling\u003c\/li\u003e \u003cli\u003eDNA Replication-Dependent Chromatin Assembly System\u003c\/li\u003e \u003cli\u003eCygnets: Intracellular Guanosine 3’,5-Cycli Monophosphate Sensing in Primary Cells Using Fluorescence Energy Transfer\u003c\/li\u003e \u003cli\u003eCa2+ as a Second Messenger: New Reporters for Calcium (Cameleons and Camgaroos)\u003c\/li\u003e \u003cli\u003eRatiometric Pericam\u003c\/li\u003e \u003cli\u003eFuorescent Indicators for Imaging Protein Phosphorylation in Single Living Cells\u003c\/li\u003e \u003cli\u003eIn Situ Electroporation of Radioactive Nucleotides: Assessment of Ras Activity or 32P Labeling of Cellular Proteins\u003c\/li\u003e \u003cli\u003eDissecting Pathways\u003cbr\u003e in Situ Electroporation for the Study of Signal Transduction and Gap Junctional Communication\u003c\/li\u003e \u003cli\u003eDetection of Protein-Protein Interactions in vivo Using Cyan and Yellow Fluorescent Proteins\u003c\/li\u003e \u003cli\u003eTracking Individual Chromosomes with Integrated Arrays of Iacop Sites and GFP-laci Repressor: Analyzing Position and Dynamics of Chromosomal Loci in Saccharomyces cerevisiae\u003c\/li\u003e \u003cli\u003eMicrotubule Motility Assays \u003c\/li\u003e \u003cli\u003eIn vitro Assays for Mitotic Spindle Assembly and Function\u003c\/li\u003e \u003cli\u003eIn vitro Motility Assays with Actin\u003c\/li\u003e \u003cli\u003eUse of Brain Cytosolic Extracts for Studying Actin-Based Motility of Listeria monocytogenes\u003c\/li\u003e \u003cli\u003ePedestal Formation by Pathogenic Escherichia coli: A Model System for Studying Signal Transduction to the Actin Cytoskeleton\u003c\/li\u003e \u003cli\u003eListeria monocytogenes: Techniques to Analyze Bacterial Infection in vitro\u003c\/li\u003e \u003cli\u003eMeasurement of Cellular Contractile Forces Using Patterned Elastomer\u003c\/li\u003e\n\u003c\/ol\u003e\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eSubject Areas: Cellular biology [\u003ca title=\"See our other books on Cellular biology\" href=\"https:\/\/freshlyprintedbooks.co.uk\/search?q=%22Cellular%20biology%20%5Bcytology%5D%20%5BPSF%5D%22\"\u003ecytology PSF\u003c\/a\u003e]\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\u003c\/font\u003e","brand":"Academic Press","offers":[{"title":"Default Title","offer_id":46649307791640,"sku":"9780123751522","price":55.36,"currency_code":"GBP","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0730\/2037\/5320\/products\/9780123751522.jpg?v=1695013967","url":"https:\/\/freshlyprintedbooks.co.uk\/products\/cell-biology-assays-essential-methods-paperback-9780123751522","provider":"Freshly Printed Books","version":"1.0","type":"link"}