{"product_id":"biophysical-chemical-and-functional-probes-of-rna-structure-interactions-and-folding-part-b-hardback-9780123809223","title":"Biophysical, Chemical, and Functional Probes of RNA Structure, Interactions and Folding: Part B (Hardback) 9780123809223","description":"\u003cfont face=\"Georgia\"\u003e\r\n\u003cp\u003e\u003cfont size=\"6\"\u003eBiophysical, Chemical, and Functional Probes of RNA Structure, Interactions and Folding: Part B\u003c\/font\u003e\u003cbr\u003e\r\n\r\n\r\n\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003e\u003cp\u003eTime-tested and innovative methods aimed at helping researchers predict protein structure\u003c\/p\u003e\u003c\/em\u003e\u003c\/p\u003e\r\n\r\n\r\n\u003cp\u003e\u003cfont size=\"4\"\u003eDaniel Herschlag (Edited by)\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e9780123809223, Elsevier Science\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eHardback, published 21 December 2009\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e502 pages\u003cbr\u003e22.9 x 15.1 x 3 cm, 1.03 kg\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\r\n\r\n\r\n\u003cp align=\"justify\"\u003e\u003cstrong\u003e\u003cfont size=\"3\"\u003eThis MIE volume provides laboratory techniques that aim to predict the structure of a protein which can have tremendous implications ranging from drug design, to cellular pathways and their dynamics, to viral entry into cells.\u003c\/font\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e\u003cp\u003eA. Chemical and Enzymatic Footprinting of RNA Structure 1. Equilibrium hydroxyl radical footprinting 2. Bench-top time-resolved hydroxyl radical footprinting 3. Analysis of hydroxyl radical footprinting gels ‘SAFA’ 4. Kinetic modeling of reaction pathways from hydroxyl radical data 5. \"BABE\" mapping of protein\/RNA position 6. Multiplexed….(MOHCA) 7. SHAPE 8. \"In-line Probing\"  9. NAIM 10. Other NAIM 11. Purification of T7 RNA Polymerase 12. Purification of T4 RNA ligase 13. In vitro transcription of RNA 14. 32P-labeling of RNA B. BIOPHYSICAL TECHNIQUES 15. Fluorescent labeling of RNAs 16. Assembly of complex RNAs by ‘Moore-Sharp’ ligations 17. Assembly of complex RNAs by ‘Moore-Sharp’ ligations  18. General considerations for smFRET with RNA samples 19. Ion counting 20. ASAXS  21. NLPB 22. smNLPB 23. Gel mobility mapping of junction structure 24. Temperature gradient gels 25. Melting studies 26. Co-transcriptional folding studies 27. Activity assays to follow folding processes 28. 2AP fluorescence 29. EPR measurements of RNA dynamics 30. EPR measurements of RNA dynamics 31. FPA measurements of RNA dynamics 32. EPR methods to study specific metal ion binding sites in RNA 33. Thermodynamic study of site-specific metal ion binding sites in RNA 34. Oligonucleotide hybridization studies of RNA folding pathways 35. Native gel shifts 36. Tiling arrays to assess RNA structure 37. EPR distance measurements in RNA 38. RNA folding in vivo 39. Cleavage of RNAs with ‘restriction DNAzymes’ \u003c\/p\u003e\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eSubject Areas: Molecular biology [\u003ca title=\"See our other books on Molecular biology\" href=\"https:\/\/freshlyprintedbooks.co.uk\/search?q=%22Molecular%20biology%20%5BPSD%5D%22\"\u003ePSD\u003c\/a\u003e], Biochemistry [\u003ca title=\"See our other books on Biochemistry\" href=\"https:\/\/freshlyprintedbooks.co.uk\/search?q=%22Biochemistry%20%5BPSB%5D%22\"\u003ePSB\u003c\/a\u003e]\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\u003c\/font\u003e","brand":"Academic Press","offers":[{"title":"Default Title","offer_id":46649311789336,"sku":"9780123809223","price":96.39,"currency_code":"GBP","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0730\/2037\/5320\/products\/9780123809223_5f11eab7-0823-4880-b9d8-705cbc8cad5b.jpg?v=1695014007","url":"https:\/\/freshlyprintedbooks.co.uk\/products\/biophysical-chemical-and-functional-probes-of-rna-structure-interactions-and-folding-part-b-hardback-9780123809223","provider":"Freshly Printed Books","version":"1.0","type":"link"}