{"product_id":"aptamers-in-bioanalysis-hardback-9780470148303","title":"Aptamers in Bioanalysis (Hardback) 9780470148303","description":"\u003cfont face=\"Georgia\"\u003e\r\n\u003cp\u003e\u003cfont size=\"6\"\u003eAptamers in Bioanalysis\u003c\/font\u003e\u003cbr\u003e\r\n\r\n\r\n\r\n\r\n\r\n\u003c\/p\u003e\n\u003cp\u003e\u003cfont size=\"4\"\u003eM. Mascini (Author)\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e9780470148303, Wiley\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eHardback, published 3 April 2009\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e364 pages\u003cbr\u003e24.3 x 16.3 x 2.3 cm, 0.626 kg\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\r\n\u003cp align=\"justify\"\u003e\u003cem\u003e\u003cfont size=\"3\"\u003e?This book provides descriptions of many applications of aptamers for biosensing, and for this reason, it is very useful and an interesting read.? (\u003ci\u003eJACS\u003c\/i\u003e , August 2009)\u003c\/font\u003e\u003c\/em\u003e\u003c\/p\u003e\r\n\r\n\u003cp align=\"justify\"\u003e\u003cstrong\u003e\u003cfont size=\"3\"\u003eThis is the first book to detail bioanalytical technologies and methods that have been developed using aptamers in analytical, medical, environmental, and food science applications. After an introduction to aptamers, aptamer targets, and their general uses, it discusses different applications with particular attention to the comparison between aptamer-based biosensors and methods versus the corresponding immunosensors. Examples of aptamer-based diagnostic techniques include whole-cell protein profiling (proteomics) and medical diagnostics for the distinction of diseased versus healthy states. This is a core reference for analytical chemists, electrochemists, pharmaceutical\/medicinal chemists, biotechnologists, and others.\u003c\/font\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e\u003cp\u003ePreface xi\u003c\/p\u003e \u003cp\u003eContributors xv\u003c\/p\u003e \u003cp\u003e\u003cb\u003eI Introduction\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Aptamers: Ligands for All Reasons 3\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eJean-Jacques Toulmé, Jean-Pierre Daguer, and Eric Dausse\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e1.1 Introduction 3\u003c\/p\u003e \u003cp\u003e1.2 The Power of Selection and Aptamer Refinement 5\u003c\/p\u003e \u003cp\u003e1.3 The Chemistry Drives the Shape 7\u003c\/p\u003e \u003cp\u003e1.4 Aptaregulators 11\u003c\/p\u003e \u003cp\u003e1.5 Aptasensors 15\u003c\/p\u003e \u003cp\u003e1.6 Prospects 18\u003c\/p\u003e \u003cp\u003eReferences 20\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 Selex and Its Recent Optimizations 31\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eBeate Strehlitz and Regina Stoltenburg\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e2.1 Introduction 31\u003c\/p\u003e \u003cp\u003e2.2 Aptamers and Their Selection by SELEX 32\u003c\/p\u003e \u003cp\u003e2.3 Modifications of SELEX Technology 35\u003c\/p\u003e \u003cp\u003e2.4 Advantages and Limitations of Aptamers and Their Selection Technology 41\u003c\/p\u003e \u003cp\u003e2.5 Applications of Aptamers Being Developed for the Market 43\u003c\/p\u003e \u003cp\u003e2.6 Future Perspectives 45\u003c\/p\u003e \u003cp\u003eReferences 50\u003c\/p\u003e \u003cp\u003e\u003cb\u003eII Biosensors\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Electrochemical Aptasensors 63\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eItamar Willner and Maya Zayats\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e3.1 Introduction 63\u003c\/p\u003e \u003cp\u003e3.2 Electrochemical Aptasensor Based on Redox-Active Aptamer Monolayers Linked to Electrodes 66\u003c\/p\u003e \u003cp\u003e3.3 Enzyme-Based Amplified Electrochemical Aptasensors 69\u003c\/p\u003e \u003cp\u003e3.4 Amplified Electrochemical Aptasensors Based on Nanoparticles 72\u003c\/p\u003e \u003cp\u003e3.5 Label-Free Electrochemical Aptasensors 75\u003c\/p\u003e \u003cp\u003e3.6 Field-Effect Transistor–Based Aptasensors 78\u003c\/p\u003e \u003cp\u003e3.7 Conclusions and Perspectives 81\u003c\/p\u003e \u003cp\u003eReferences 81\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 Aptamers: Hybrids Between Nature And Technology 87\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eMoritz K. Beissenhirtz, Eik Leupold, Walter Stöcklein, Ulla Wollenberger, Oliver Pänke, Fred Lisdat, and Frieder W. Scheller\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e4.1 Introduction 87\u003c\/p\u003e \u003cp\u003e4.2 Specific Features of Aptamers 88\u003c\/p\u003e \u003cp\u003e4.3 Electrochemical Detection of Nucleic Acids 88\u003c\/p\u003e \u003cp\u003e4.4 Cytochrome c Binding by Aptamers 90\u003c\/p\u003e \u003cp\u003e4.5 DNA Machines and Aptamers 92\u003c\/p\u003e \u003cp\u003eReferences 98\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 Detection of Protein–aptamer Interactions By Means of Electrochemical Indicators And Transverse Shear Mode Method 101\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eTibor Hianik\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e5.1 Introduction 101\u003c\/p\u003e \u003cp\u003e5.2 Immobilization of Aptamers on a Solid Support 102\u003c\/p\u003e \u003cp\u003e5.3 Detection of Aptamer–Ligand Interactions 104\u003c\/p\u003e \u003cp\u003e5.3.1 Electrochemical Methods 105\u003c\/p\u003e \u003cp\u003e5.3.2 Acoustic Methods 117\u003c\/p\u003e \u003cp\u003e5.4 Conclusions 124\u003c\/p\u003e \u003cp\u003eReferences 125\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Biosensors Using the Aptameric Enzyme Subunit: the Use of Aptamers in the Allosteric Control of Enzymes 129\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eKazunori Ikebukuro, Wataru Yoshida, and Koji Sode\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e6.1 Aptamers as Molecular Recognition Elements of Biosensors 129\u003c\/p\u003e \u003cp\u003e6.1.1 Comparing Aptamers to Antibodies 129\u003c\/p\u003e \u003cp\u003e6.1.2 Signaling Aptamers 131\u003c\/p\u003e \u003cp\u003e6.2 Homogeneous Sensing 133\u003c\/p\u003e \u003cp\u003e6.2.1 Biosensor Systems That Do Not Require Bound–Free Separation 133\u003c\/p\u003e \u003cp\u003e6.2.2 Aptameric Enzyme Subunit 133\u003c\/p\u003e \u003cp\u003e6.3 Evolution-mimicking Algorithm for the Improvement of Aptamers 136\u003c\/p\u003e \u003cp\u003eReferences 137\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Nanomaterial-based Label-free Aptasensors 139\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eKagan Kerman and Eiichi Tamiya\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e7.1 Introduction 139\u003c\/p\u003e \u003cp\u003e7.2 Label-Free Electrochemical Aptasensors 139\u003c\/p\u003e \u003cp\u003e7.3 Field-Effect Transistor–Based Aptasensors 143\u003c\/p\u003e \u003cp\u003e7.4 Label-Free Aptasensors Based on Localized Surface Plasmon Resonance 147\u003c\/p\u003e \u003cp\u003e7.5 Forthcoming Challenges and Concluding Remarks 151\u003c\/p\u003e \u003cp\u003eReferences 151\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 Aptamer-based Bioanalytical Assays: Amplification Strategies 159\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eSara Tombelli, Maria Minunni, and Marco Mascini\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e8.1 Introduction 159\u003c\/p\u003e \u003cp\u003e8.2 Bioanalytical Assays Based on Aptamer-Functionalized Nanoparticles 160\u003c\/p\u003e \u003cp\u003e8.3 Aptamers and Quantum Dot–Based Assays 164\u003c\/p\u003e \u003cp\u003e8.4 Aptazymes and Aptamer-Based Machines 168\u003c\/p\u003e \u003cp\u003e8.5 Polymerase Chain Reaction as an Amplification Method in Aptamer-Based Assays 173\u003c\/p\u003e \u003cp\u003e8.6 Conclusions 176\u003c\/p\u003e \u003cp\u003eReferences 177\u003c\/p\u003e \u003cp\u003e\u003cb\u003eIII Applications\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9 Kinetic Capillary Electrophoresis for Selection, Characterization, and Analytical Utilization of Aptamers 183\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eSergey N. Krylov\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e9.1 Introduction 183\u003c\/p\u003e \u003cp\u003e9.1.1 Kinetic Capillary Electrophoresis 183\u003c\/p\u003e \u003cp\u003e9.1.2 The Concept of NECEEM and ECEEM 185\u003c\/p\u003e \u003cp\u003e9.2 Selection of Aptamers Using KCE Methods for Partitioning and Affinity Control 188\u003c\/p\u003e \u003cp\u003e9.2.1 NECEEM-Based Selection of Aptamers 188\u003c\/p\u003e \u003cp\u003e9.2.2 ECEEM-Based Selection of Aptamers 197\u003c\/p\u003e \u003cp\u003e9.2.3 Optimization of PCR 198\u003c\/p\u003e \u003cp\u003e9.2.4 Future of KCE Methods for Aptamer Selection 200\u003c\/p\u003e \u003cp\u003e9.3 Measurements of Binding Parameters of Target–Aptamer Interaction by KCE Methods 200\u003c\/p\u003e \u003cp\u003e9.3.1 Foundation 200\u003c\/p\u003e \u003cp\u003e9.3.2 Temperature Control Inside the Capillary 202\u003c\/p\u003e \u003cp\u003e9.3.3 Examples 203\u003c\/p\u003e \u003cp\u003e9.4 Quantitative Affinity Analysis of a Target Using Aptamer as an Affinity Probe 205\u003c\/p\u003e \u003cp\u003e9.4.1 Foundation 205\u003c\/p\u003e \u003cp\u003e9.4.2 Example 208\u003c\/p\u003e \u003cp\u003e9.5 Conclusions 209\u003c\/p\u003e \u003cp\u003eReferences 210\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10 Aptamers for Separation of Enantiomers 213\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eCorinne Ravelet and Eric Peyrin\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e10.1 Introduction 213\u003c\/p\u003e \u003cp\u003e10.2 Generation and Properties of Enantioselective Aptamers 214\u003c\/p\u003e \u003cp\u003e10.3 Immobilized Aptamers for Enantiomeric Separation by Liquid Chromatography 215\u003c\/p\u003e \u003cp\u003e10.3.1 Stationary-Phase Preparation and Column Packing 216\u003c\/p\u003e \u003cp\u003e10.3.2 DNA Aptamer-Based CSPs 216\u003c\/p\u003e \u003cp\u003e10.3.3 RNA Aptamer-Based CSPs and the Mirror-Image Strategy 217\u003c\/p\u003e \u003cp\u003e10.3.4 Class-Specific Aptamer-Based CSPs 219\u003c\/p\u003e \u003cp\u003e10.4 Aptamers for Analysis of Enantiomers by Capillary Electrophoresis 221\u003c\/p\u003e \u003cp\u003e10.4.1 Aptamers as Chiral Additives in the Background Electrolyte for CE Enantiomeric Separation 221\u003c\/p\u003e \u003cp\u003e10.4.2 Aptamers for the Design of an Affinity CE-Based Enantioselective Competitive Assay 223\u003c\/p\u003e \u003cp\u003e10.5 Conclusions 226\u003c\/p\u003e \u003cp\u003eReferences 226\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11 Aptamer-modified Surfaces for Affinity Capture and Detection of Proteins in Capillary Electrophoresis and Maldi–mass Spectrometry 229\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eLinda B. McGown\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e11.1 Introduction 229\u003c\/p\u003e \u003cp\u003e11.2 Aptamer-Modified Capillaries in Affinity Capillary Electrophoresis 230\u003c\/p\u003e \u003cp\u003e11.3 Aptamer-Modified Surfaces for Affinity MALDI-MS 232\u003c\/p\u003e \u003cp\u003e11.3.1 Overview 232\u003c\/p\u003e \u003cp\u003e11.3.2 Affinity MALDI-MS of Thrombin 233\u003c\/p\u003e \u003cp\u003e11.3.3 Affinity MALDI-MS of IgE 235\u003c\/p\u003e \u003cp\u003e11.3.4 Summary 242\u003c\/p\u003e \u003cp\u003e11.4 Beyond Aptamers: Genome-Inspired DNA Binding Ligands 242\u003c\/p\u003e \u003cp\u003eReferences 248\u003c\/p\u003e \u003cp\u003e\u003cb\u003e12 Strategy for Use of Smart Routes to Prepare Label-free Aptasensors for Bioassay Using Different Techniques 251\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eBingling Li, Hui Wei, and Shaojun Dong\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e12.1 Introduction 251\u003c\/p\u003e \u003cp\u003e12.2 Electrochemical Aptasensors 254\u003c\/p\u003e \u003cp\u003e12.2.1 POSOALF Mode 254\u003c\/p\u003e \u003cp\u003e12.2.2 PFSOALF Mode 257\u003c\/p\u003e \u003cp\u003e12.2.3 Electrochemical Impedimetric Aptasensors 257\u003c\/p\u003e \u003cp\u003e12.2.4 Electrochemical Aptasensors with Nonlabeled Redox Probes 263\u003c\/p\u003e \u003cp\u003e12.3 Fluorescent Molecular Switches 265\u003c\/p\u003e \u003cp\u003e12.3.1 POSFALF Mode 266\u003c\/p\u003e \u003cp\u003e12.3.2 PFSFALF Mode 268\u003c\/p\u003e \u003cp\u003e12.4 Colorimetry 272\u003c\/p\u003e \u003cp\u003e12.4.1 POSFALF Mode 273\u003c\/p\u003e \u003cp\u003e12.4.2 PFSFALF Mode 274\u003c\/p\u003e \u003cp\u003e12.5 Hemin–Aptamer DNAzyme-Based Aptasensor 281\u003c\/p\u003e \u003cp\u003e12.6 Liquid Chromatography, Electrochromatography, and Capillary Electrophoresis Applications 284\u003c\/p\u003e \u003cp\u003e12.7 Other Aptasensors 290\u003c\/p\u003e \u003cp\u003e12.8 Conclusions 290\u003c\/p\u003e \u003cp\u003eReferences 290\u003c\/p\u003e \u003cp\u003eIndex 299\u003c\/p\u003e\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eSubject Areas: Chemistry [\u003ca title=\"See our other books on Chemistry\" href=\"https:\/\/freshlyprintedbooks.co.uk\/search?q=%22Chemistry%20%5BPN%5D%22\"\u003ePN\u003c\/a\u003e]\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\u003c\/font\u003e","brand":"Wiley-Interscience","offers":[{"title":"Brand New","offer_id":52257119174936,"sku":"9780470148303","price":127.29,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0730\/2037\/5320\/files\/9780470148303.jpg?v=1781277095","url":"https:\/\/freshlyprintedbooks.co.uk\/products\/aptamers-in-bioanalysis-hardback-9780470148303","provider":"Freshly Printed Books","version":"1.0","type":"link"}