{"product_id":"an-introduction-to-high-content-screening-imaging-technology-assay-development-and-data-analysis-in-biology-and-drug-discovery-hardback-9780470624562","title":"An Introduction To High Content Screening; Imaging Technology, Assay Development, and Data Analysis in Biology and Drug Discovery (Hardback) 9780470624562","description":"\u003cfont face=\"Georgia\"\u003e\r\n\u003cp\u003e\u003cfont size=\"6\"\u003eAn Introduction To High Content Screening\u003c\/font\u003e\u003cbr\u003e\r\n\u003cfont size=\"5\"\u003eImaging Technology, Assay Development, and Data Analysis in Biology and Drug Discovery\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\r\n\r\n\u003cp\u003e\u003cfont size=\"4\"\u003eSteven A. Haney (Edited by), SA Haney (Author), Douglas Bowman (Edited by), Arijit Chakravarty (Edited by), Anthony Davies (Associate editor), Caroline Shamu (Associate editor)\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e9780470624562, Wiley\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eHardback, published 31 December 2014\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e360 pages\u003cbr\u003e24.4 x 16.4 x 2.4 cm, 0.603 kg\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\r\n\r\n\r\n\u003cp align=\"justify\"\u003e\u003cstrong\u003e\u003cfont size=\"3\"\u003e\u003cp\u003e\u003cbr\u003e Using a collaborative and interdisciplinary author base with experience in the pharmaceutical industry and academia, this book is a practical resource for high content (HC) techniques.\u003c\/p\u003e \u003cp\u003e• Instructs readers on the fundamentals of high content screening (HCS) techniques\u003cbr\u003e • Focuses on practical and widely-used techniques like image processing and multiparametric assays\u003cbr\u003e • Breaks down HCS into individual modules for training and connects them at the end\u003cbr\u003e • Includes a tutorial chapter that works through sample HCS assays, glossary, and detailed appendices\u003c\/p\u003e\u003c\/font\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e\u003cp\u003ePREFACE xvii\u003c\/p\u003e \u003cp\u003eCONTRIBUTORS xix\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Introduction 1\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eSteven A. Haney\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e1.1 The Beginning of High Content Screening, 1\u003c\/p\u003e \u003cp\u003e1.2 Six Skill Sets Essential for Running HCS Experiments, 4\u003c\/p\u003e \u003cp\u003e1.3 Integrating Skill Sets into a Team, 7\u003c\/p\u003e \u003cp\u003e1.4 A Few Words on Experimental Design, 8\u003c\/p\u003e \u003cp\u003e1.5 Conclusions, 9\u003c\/p\u003e \u003cp\u003eKey Points, 9\u003c\/p\u003e \u003cp\u003eFurther Reading, 10\u003c\/p\u003e \u003cp\u003eReferences, 10\u003c\/p\u003e \u003cp\u003e\u003cb\u003eSECTION I FIRST PRINCIPLES 11\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 Fluorescence and Cell Labeling 13\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eAnthony Davies and Steven A. Haney\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e2.1 Introduction, 13\u003c\/p\u003e \u003cp\u003e2.2 Anatomy of Fluorescent Probes, Labels, and Dyes, 14\u003c\/p\u003e \u003cp\u003e2.3 Stokes’ Shift and Biological Fluorophores, 15\u003c\/p\u003e \u003cp\u003e2.4 Fluorophore Properties, 16\u003c\/p\u003e \u003cp\u003e2.5 Localization of Fluorophores Within Cells, 18\u003c\/p\u003e \u003cp\u003e2.6 Multiplexing Fluorescent Reagents, 26\u003c\/p\u003e \u003cp\u003e2.7 Specialized Imaging Applications Derived from Complex Properties of Fluorescence, 27\u003c\/p\u003e \u003cp\u003e2.8 Conclusions, 30\u003c\/p\u003e \u003cp\u003eKey Points, 31\u003c\/p\u003e \u003cp\u003eFurther Reading, 31\u003c\/p\u003e \u003cp\u003eReferences, 31\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Microscopy Fundamentals 33\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eSteven A. Haney, Anthony Davies, and Douglas Bowman\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e3.1 Introducing HCS Hardware, 33\u003c\/p\u003e \u003cp\u003e3.2 Deconstructing Light Microscopy, 37\u003c\/p\u003e \u003cp\u003e3.3 Using the Imager to Collect Data, 43\u003c\/p\u003e \u003cp\u003e3.4 Conclusions, 45\u003c\/p\u003e \u003cp\u003eKey Points, 45\u003c\/p\u003e \u003cp\u003eFurther Reading, 46\u003c\/p\u003e \u003cp\u003eReferences, 46\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 Image Processing 47\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eJohn Bradley, Douglas Bowman, and Arijit Chakravarty\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e4.1 Overview of Image Processing and Image Analysis in HCS, 47\u003c\/p\u003e \u003cp\u003e4.2 What is a Digital Image?, 48\u003c\/p\u003e \u003cp\u003e4.3 “Addressing” Pixel Values in Image Analysis Algorithms, 48\u003c\/p\u003e \u003cp\u003e4.4 Image Analysis Workflow, 49\u003c\/p\u003e \u003cp\u003e4.5 Conclusions, 60\u003c\/p\u003e \u003cp\u003eKey Points, 60\u003c\/p\u003e \u003cp\u003eFurther Reading, 60\u003c\/p\u003e \u003cp\u003eReferences, 60\u003c\/p\u003e \u003cp\u003e\u003cb\u003eSECTION II GETTING STARTED 63\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 A General Guide to Selecting and Setting Up a High Content Imaging Platform 65\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eCraig Furman, Douglas Bowman, Anthony Davies, Caroline Shamu, and Steven A. Haney\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e5.1 Determining Expectations of the HCS System, 65\u003c\/p\u003e \u003cp\u003e5.2 Establishing an HC Platform Acquisition Team, 66\u003c\/p\u003e \u003cp\u003e5.3 Basic Hardware Decisions, 67\u003c\/p\u003e \u003cp\u003e5.4 Data Generation, Analysis, and Retention, 72\u003c\/p\u003e \u003cp\u003e5.5 Installation, 73\u003c\/p\u003e \u003cp\u003e5.6 Managing the System, 75\u003c\/p\u003e \u003cp\u003e5.7 Setting Up Workflows for Researchers, 77\u003c\/p\u003e \u003cp\u003e5.8 Conclusions, 78\u003c\/p\u003e \u003cp\u003eKey Points, 79\u003c\/p\u003e \u003cp\u003eFurther Reading, 79\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Informatics Considerations 81\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eJay Copeland and Caroline Shamu\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e6.1 Informatics Infrastructure for High Content Screening, 81\u003c\/p\u003e \u003cp\u003e6.2 Using Databases to Store HCS Data, 86\u003c\/p\u003e \u003cp\u003e6.3 Mechanics of an Informatics Solution, 89\u003c\/p\u003e \u003cp\u003e6.4 Developing Image Analysis Pipelines: Data Management Considerations, 95\u003c\/p\u003e \u003cp\u003e6.5 Compliance With Emerging Data Standards, 99\u003c\/p\u003e \u003cp\u003e6.6 Conclusions, 101\u003c\/p\u003e \u003cp\u003eKey Points, 102\u003c\/p\u003e \u003cp\u003eFurther Reading, 102\u003c\/p\u003e \u003cp\u003eReferences, 102\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Basic High Content Assay Development 103\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eSteven A. Haney and Douglas Bowman\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e7.1 Introduction, 103\u003c\/p\u003e \u003cp\u003e7.2 Initial Technical Considerations for Developing a High Content Assay, 103\u003c\/p\u003e \u003cp\u003e7.3 A Simple Protocol to Fix and Stain Cells, 107\u003c\/p\u003e \u003cp\u003e7.4 Image Capture and Examining Images, 109\u003c\/p\u003e \u003cp\u003e7.5 Conclusions, 111\u003c\/p\u003e \u003cp\u003eKey Points, 112\u003c\/p\u003e \u003cp\u003eFurther Reading, 112\u003c\/p\u003e \u003cp\u003eReference, 112\u003c\/p\u003e \u003cp\u003e\u003cb\u003eSECTION III ANALYZING DATA 113\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 Designing Metrics for High Content Assays 115\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eArijit Chakravarty, Steven A. Haney, and Douglas Bowman\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e8.1 Introduction: Features, Metrics, Results, 115\u003c\/p\u003e \u003cp\u003e8.2 Looking at Features, 116\u003c\/p\u003e \u003cp\u003e8.3 Metrics and Results: The Metric is the Message, 120\u003c\/p\u003e \u003cp\u003e8.4 Types of High Content Assays and Their Metrics, 121\u003c\/p\u003e \u003cp\u003e8.5 Metrics to Results: Putting it all Together, 126\u003c\/p\u003e \u003cp\u003e8.6 Conclusions, 128\u003c\/p\u003e \u003cp\u003eKey Points, 128\u003c\/p\u003e \u003cp\u003eFurther Reading, 129\u003c\/p\u003e \u003cp\u003eReferences, 129\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9 Analyzing Well-Level Data 131\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eSteven A Haney and John Ringeling\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e9.1 Introduction, 131\u003c\/p\u003e \u003cp\u003e9.2 Reviewing Data, 132\u003c\/p\u003e \u003cp\u003e9.3 Plate and Control Normalizations of Data, 134\u003c\/p\u003e \u003cp\u003e9.4 Calculation of Assay Statistics, 135\u003c\/p\u003e \u003cp\u003e9.5 Data Analysis: Hit Selection, 138\u003c\/p\u003e \u003cp\u003e9.6 IC 50 Determinations, 139\u003c\/p\u003e \u003cp\u003e9.7 Conclusions, 143\u003c\/p\u003e \u003cp\u003eKey Points, 143\u003c\/p\u003e \u003cp\u003eFurther Reading, 143\u003c\/p\u003e \u003cp\u003eReferences, 144\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10 Analyzing Cell-Level Data 145\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eSteven A. Haney, Lin Guey, and Arijit Chakravarty\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e10.1 Introduction, 145\u003c\/p\u003e \u003cp\u003e10.2 Understanding General Statistical Terms and Concepts, 146\u003c\/p\u003e \u003cp\u003e10.3 Examining Data, 149\u003c\/p\u003e \u003cp\u003e10.4 Developing a Data Analysis Plan, 155\u003c\/p\u003e \u003cp\u003e10.5 Cell-Level Data Analysis: Comparing Distributions Through Inferential Statistics, 158\u003c\/p\u003e \u003cp\u003e10.6 Analyzing Normal (or Transformed) Data, 159\u003c\/p\u003e \u003cp\u003e10.7 Analyzing Non-Normal Data, 160\u003c\/p\u003e \u003cp\u003e10.8 When to Call For Help, 162\u003c\/p\u003e \u003cp\u003e10.9 Conclusions, 162\u003c\/p\u003e \u003cp\u003eKey Points, 162\u003c\/p\u003e \u003cp\u003eFurther Reading, 163\u003c\/p\u003e \u003cp\u003eReferences, 163\u003c\/p\u003e \u003cp\u003e\u003cb\u003eSECTION IV ADVANCED WORK 165\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11 Designing Robust Assays 167\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eArijit Chakravarty, Douglas Bowman, Anthony Davies, Steven A. Haney, and Caroline Shamu\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e11.1 Introduction, 167\u003c\/p\u003e \u003cp\u003e11.2 Common Technical Issues in High Content Assays, 167\u003c\/p\u003e \u003cp\u003e11.3 Designing Assays to Minimize Trouble, 172\u003c\/p\u003e \u003cp\u003e11.4 Looking for Trouble: Building in Quality Control, 177\u003c\/p\u003e \u003cp\u003e11.5 Conclusions, 179\u003c\/p\u003e \u003cp\u003eKey Points, 180\u003c\/p\u003e \u003cp\u003eFurther Reading, 180\u003c\/p\u003e \u003cp\u003eReferences, 180\u003c\/p\u003e \u003cp\u003e\u003cb\u003e12 Automation and Screening 181\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eJohn Ringeling, John Donovan, Arijit Chakravarty, Anthony Davies, Steven A Haney, Douglas Bowman, and Ben Knight\u003cbr\u003e\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e12.1 Introduction, 181\u003c\/p\u003e \u003cp\u003e12.2 Some Preliminary Considerations, 181\u003c\/p\u003e \u003cp\u003e12.3 Laboratory Options, 183\u003c\/p\u003e \u003cp\u003e12.4 The Automated HCS Laboratory, 186\u003c\/p\u003e \u003cp\u003e12.5 Conclusions, 192\u003c\/p\u003e \u003cp\u003eKey Points, 192\u003c\/p\u003e \u003cp\u003eFurther Reading, 193\u003c\/p\u003e \u003cp\u003e\u003cb\u003e13 High Content Analysis for Tissue Samples 195\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eKristine Burke, Vaishali Shinde, Alice McDonald, Douglas Bowman, and Arijit Chakravarty\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e13.1 Introduction, 195\u003c\/p\u003e \u003cp\u003e13.2 Design Choices in Setting Up a High Content Assay in Tissue, 196\u003c\/p\u003e \u003cp\u003e13.3 System Configuration: Aspects Unique to Tissue-Based HCS, 199\u003c\/p\u003e \u003cp\u003e13.4 Data Analysis, 203\u003c\/p\u003e \u003cp\u003e13.5 Conclusions, 207\u003c\/p\u003e \u003cp\u003eKey Points, 207\u003c\/p\u003e \u003cp\u003eFurther Reading, 207\u003c\/p\u003e \u003cp\u003eReferences, 208\u003c\/p\u003e \u003cp\u003e\u003cb\u003eSECTION V HIGH CONTENT ANALYTICS 209\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e14 Factoring and Clustering High Content Data 211\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eSteven A. Haney\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e14.1 Introduction, 211\u003c\/p\u003e \u003cp\u003e14.2 Common Unsupervised Learning Methods, 212\u003c\/p\u003e \u003cp\u003e14.3 Preparing for an Unsupervised Learning Study, 218\u003c\/p\u003e \u003cp\u003e14.4 Conclusions, 228\u003c\/p\u003e \u003cp\u003eKey Points, 228\u003c\/p\u003e \u003cp\u003eFurther Reading, 228\u003c\/p\u003e \u003cp\u003eReferences, 229\u003c\/p\u003e \u003cp\u003e\u003cb\u003e15 Supervised Machine Learning 231\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eJeff Palmer and Arijit Chakravarty\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e15.1 Introduction, 231\u003c\/p\u003e \u003cp\u003e15.2 Foundational Concepts, 232\u003c\/p\u003e \u003cp\u003e15.3 Choosing a Machine Learning Algorithm, 234\u003c\/p\u003e \u003cp\u003e15.4 When Do You Need Machine Learning, and How Do You Use IT?, 243\u003c\/p\u003e \u003cp\u003e15.5 Conclusions, 244\u003c\/p\u003e \u003cp\u003eKey Points, 244\u003c\/p\u003e \u003cp\u003eFurther Reading, 244\u003c\/p\u003e \u003cp\u003e\u003cb\u003eAppendix A Websites and Additional Information on Instruments, Reagents, and Instruction 247\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003eAppendix B A Few Words About One Letter: Using R to Quickly\u003c\/b\u003e \u003cb\u003eAnalyze HCS Data 249\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eSteven A. Haney\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003eB.1 Introduction, 249\u003c\/p\u003e \u003cp\u003eB.2 Setting Up R, 250\u003c\/p\u003e \u003cp\u003eB.3 Analyzing Data in R, 253\u003c\/p\u003e \u003cp\u003eB.4 Where to Go Next, 261\u003c\/p\u003e \u003cp\u003eFurther Reading, 263\u003c\/p\u003e \u003cp\u003e\u003cb\u003eAppendix C Hypothesis Testing for High Content Data: A Refresher 265\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eLin Guey and Arijit Chakravarty\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003eC.1 Introduction, 265\u003c\/p\u003e \u003cp\u003eC.2 Defining Simple Hypothesis Testing, 266\u003c\/p\u003e \u003cp\u003eC.3 Simple Statistical Tests to Compare Two Groups, 269\u003c\/p\u003e \u003cp\u003eC.4 Statistical Tests on Groups of Samples, 276\u003c\/p\u003e \u003cp\u003eC.5 Introduction to Regression Models, 280\u003c\/p\u003e \u003cp\u003eC.6 Conclusions, 285\u003c\/p\u003e \u003cp\u003eKey Concepts, 286\u003c\/p\u003e \u003cp\u003eFurther Reading, 286\u003c\/p\u003e \u003cp\u003eGLOSSARY 287\u003c\/p\u003e \u003cp\u003eTUTORIAL 295\u003c\/p\u003e \u003cp\u003eINDEX 323\u003c\/p\u003e\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eSubject Areas: Chemistry [\u003ca title=\"See our other books on Chemistry\" href=\"https:\/\/freshlyprintedbooks.co.uk\/search?q=%22Chemistry%20%5BPN%5D%22\"\u003ePN\u003c\/a\u003e]\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\u003c\/font\u003e","brand":"Wiley","offers":[{"title":"Brand New","offer_id":52276370374936,"sku":"9780470624562","price":96.59,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0730\/2037\/5320\/files\/9780470624562.jpg?v=1781368109","url":"https:\/\/freshlyprintedbooks.co.uk\/products\/an-introduction-to-high-content-screening-imaging-technology-assay-development-and-data-analysis-in-biology-and-drug-discovery-hardback-9780470624562","provider":"Freshly Printed Books","version":"1.0","type":"link"}